Characterized mechanism of α-mangostin-induced cell death: Caspase-independent apoptosis with release of endonuclease-G from mitochondria and increased miR-143 expression in human colorectal cancer DLD-1 cells

Yoshihito Nakagawa, Munekazu Iinuma, Tomoki Naoe, Yoshinori Nozawa, Yukihiro Akao

研究成果: ジャーナルへの寄稿学術論文査読

162 被引用数 (Scopus)

抄録

α-Mangostin, a xanthone from the pericarps of mangosteen (Garcinia mangostana Linn.), was evaluated for in vitro cytotoxicity against human colon cancer DLD-1 cells. The number of viable cells was consistently decreased by the treatment with α-mangostin at more than 20 μM. The cytotoxic effect of 20 μM α-mangostin was found to be mainly due to apoptosis, as indicated by morphological findings. Western blotting, the results of an apoptosis inhibition assay using caspase inhibitors, and the examination of caspase activity did not demonstrate the activation of any of the caspases tested. However, endonuclease-G released from mitochondria with the decreased mitochondrial membrane potential was shown. The levels of phospho-Erk1/2 were increased in the early phase until 1 h after the start of treatment and thereafter decreased, and increased again in the late phase. On the other hand, the level of phospho-Akt was sharply reduced with the process of apoptosis after 6 h of treatment. Interestingly, the level of microRNA-143, which negatively regulates Erk5 at translation, gradually increased until 24 h following the start of treatment. We also examined the synergistic growth suppression in DLD-1 cells by the combined treatment of the cells with α-mangostin and 5-FU which is one of the most effective chemotherapeutic agents for colorectal adenocarcinoma. The co-treatment with α-mangostin and 5-FU, both at 2.5 μM, augmented growth inhibition compared with the treatment with 5 μM of α-mangostin or 5 μM 5-FU alone. These findings indicate unique mechanisms of α-mangostin-induced apoptosis and its action as an effective chemosensitizer.

本文言語英語
ページ(範囲)5620-5628
ページ数9
ジャーナルBioorganic and Medicinal Chemistry
15
16
DOI
出版ステータス出版済み - 15-08-2007
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子医療
  • 分子生物学
  • 薬科学
  • 創薬
  • 臨床生化学
  • 有機化学

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