Chemogenetic activation of nigrostriatal dopamine neurons in freely moving common marmosets

Koki Mimura, Yuji Nagai, Ken ichi Inoue, Jumpei Matsumoto, Yukiko Hori, Chika Sato, Kei Kimura, Takashi Okauchi, Toshiyuki Hirabayashi, Hisao Nishijo, Noriaki Yahata, Masahiko Takada, Tetsuya Suhara, Makoto Higuchi, Takafumi Minamimoto

研究成果: ジャーナルへの寄稿学術論文査読

21 被引用数 (Scopus)

抄録

To interrogate particular neuronal pathways in nonhuman primates under natural and stress-free conditions, we applied designer receptors exclusively activated by designer drugs (DREADDs) technology to common marmosets. We injected adeno-associated virus vectors expressing the excitatory DREADD hM3Dq into the unilateral substantia nigra (SN) in four marmosets. Using multi-tracer positron emission tomography imaging, we detected DREADD expression in vivo, which was confirmed in nigrostriatal dopamine neurons by immunohistochemistry, as well as by assessed activation of the SN following agonist administration. The marmosets rotated in a contralateral direction relative to the activated side 30–90 min after consuming food containing the highly potent DREADD agonist deschloroclozapine (DCZ) but not on the following days without DCZ. These results indicate that non-invasive and reversible DREADD manipulation will extend the utility of marmosets as a primate model for linking neuronal activity and natural behavior in various contexts.

本文言語英語
論文番号103066
ジャーナルiScience
24
9
DOI
出版ステータス出版済み - 24-09-2021
外部発表はい

All Science Journal Classification (ASJC) codes

  • 一般

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