TY - JOUR
T1 - Clinical significance of direct fast scarlet staining on the diagnosis of eosinophilic colitis
T2 - A comparative study focusing on the eosinophil degranulation in colonic mucosal tissue
AU - Ikeda, Mina
AU - Kato, Hiroyuki
AU - Arakawa, Satoshi
AU - Kobayashi, Takashi
AU - Hashimoto, Senju
AU - Katano, Yoshiaki
AU - Inada, Ken ichi
AU - Kiriyama, Yuka
AU - Ishihara, Takuma
AU - Yamamoto, Satoshi
AU - Asano, Yukio
AU - Horiguchi, Akihiko
N1 - Publisher Copyright:
© 2024 Japanese Society of Pathology and John Wiley & Sons Australia, Ltd.
PY - 2024/11
Y1 - 2024/11
N2 - This study aimed to validate the DFS (direct fast scarlet) staining in the diagnosis of EC (eosinophilic colitis). The study included 50 patients with EC and 60 with control colons. Among the 60 control samples, 39 and 21 were collected from the ascending and descending colons, respectively. We compared the median number of eosinophils and frequency of eosinophil degranulation by HE (hematoxylin and eosin) and DFS staining between the EC and control groups. In the right hemi-colon, eosinophil count by HE was useful in distinguishing between EC and control (41.5 vs. 26.0 cells/HPF, p < 0.001), but the ideal cutoff value is 27.5 cells/HPF (high-power field). However, this method is not useful in the left hemi-colon (12.5 vs. 13.0 cells/HPF, p = 0.990). The presence of degranulation by DFS allows us to distinguish between the groups even in the left hemi-colon (58% vs. 5%, p < 0.001). DFS staining also enabled a more accurate determination of degranulation than HE. According to the current standard to diagnose EC (count by HE staining ≥20 cells/HPF), mucosal sampling from left hemi-colon is problematic since the number of eosinophils could not be increased even in EC. Determination of degranulated eosinophils by DFS may potentiate the diagnostic performance even in such conditions.
AB - This study aimed to validate the DFS (direct fast scarlet) staining in the diagnosis of EC (eosinophilic colitis). The study included 50 patients with EC and 60 with control colons. Among the 60 control samples, 39 and 21 were collected from the ascending and descending colons, respectively. We compared the median number of eosinophils and frequency of eosinophil degranulation by HE (hematoxylin and eosin) and DFS staining between the EC and control groups. In the right hemi-colon, eosinophil count by HE was useful in distinguishing between EC and control (41.5 vs. 26.0 cells/HPF, p < 0.001), but the ideal cutoff value is 27.5 cells/HPF (high-power field). However, this method is not useful in the left hemi-colon (12.5 vs. 13.0 cells/HPF, p = 0.990). The presence of degranulation by DFS allows us to distinguish between the groups even in the left hemi-colon (58% vs. 5%, p < 0.001). DFS staining also enabled a more accurate determination of degranulation than HE. According to the current standard to diagnose EC (count by HE staining ≥20 cells/HPF), mucosal sampling from left hemi-colon is problematic since the number of eosinophils could not be increased even in EC. Determination of degranulated eosinophils by DFS may potentiate the diagnostic performance even in such conditions.
KW - degranulation
KW - diagnostic performance
KW - direct fast scarlet staining
KW - eosinophilic colitis
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U2 - 10.1111/pin.13475
DO - 10.1111/pin.13475
M3 - Article
C2 - 39259048
AN - SCOPUS:85203695439
SN - 1320-5463
VL - 74
SP - 632
EP - 640
JO - Pathology International
JF - Pathology International
IS - 11
ER -