Clonality analysis of granulocytes and T lymphocytes in healthy females by the PCR-based HUMARA method

Yousuke Nakahara, Hitoshi Suzuki, Haruhiko Ohashi, Sonoko Hatano, Akihiro Tomita, Tomohiro Kinoshita, Takashi Murate, Hidehiko Saito, Tomomitsu Hotta

研究成果: Article

12 引用 (Scopus)

抄録

Clonality analysis utilizing X-chromosome inactivation has been used in the study of various diseases, including hematological malignancies. The human androgen receptor gene (HUMARA) assay is the newest of such methods, and the majority of the female population can be assessed by this relatively simple procedure. One problem in using these clonality analysis methods, however, is that there may be significant variation in Lyonization in blood cells in normal individuals. To determine the diversity in X-chromosome methylation patterns, which reflect Lyonization, assessed by the HUMARA assay in the supposedly normal population, we analyzed granulocytes and T cells from 97 relatively young (18- to 35-year-old) healthy female volunteers. We found that the methylation patterns in the two HUMARA alleles were distributed even more widely, both in granuloctyes and in T cells, than previously reported with other methods. We also found that the deviation of methylation in granulocytes and T cells was well correlated. Thus, we conclude that appropriate controls from the same individuals, such as T cells in the case of stem cell disorders, should always be employed to conclusively determine whether certain cells of hematopoietic origin are clonal.

元の言語English
ページ(範囲)237-243
ページ数7
ジャーナルInternational Journal of Hematology
69
発行部数4
出版物ステータスPublished - 01-12-1999
外部発表Yes

Fingerprint

Granulocytes
X Chromosome Inactivation
Methylation
T-Lymphocytes
Polymerase Chain Reaction
X Chromosome
Hematologic Neoplasms
Population
Blood Cells
Healthy Volunteers
Stem Cells
Alleles
Genes

All Science Journal Classification (ASJC) codes

  • Hematology

これを引用

Nakahara, Y., Suzuki, H., Ohashi, H., Hatano, S., Tomita, A., Kinoshita, T., ... Hotta, T. (1999). Clonality analysis of granulocytes and T lymphocytes in healthy females by the PCR-based HUMARA method. International Journal of Hematology, 69(4), 237-243.
Nakahara, Yousuke ; Suzuki, Hitoshi ; Ohashi, Haruhiko ; Hatano, Sonoko ; Tomita, Akihiro ; Kinoshita, Tomohiro ; Murate, Takashi ; Saito, Hidehiko ; Hotta, Tomomitsu. / Clonality analysis of granulocytes and T lymphocytes in healthy females by the PCR-based HUMARA method. :: International Journal of Hematology. 1999 ; 巻 69, 番号 4. pp. 237-243.
@article{84c9839fbcaa479bb77c6e614d6812d9,
title = "Clonality analysis of granulocytes and T lymphocytes in healthy females by the PCR-based HUMARA method",
abstract = "Clonality analysis utilizing X-chromosome inactivation has been used in the study of various diseases, including hematological malignancies. The human androgen receptor gene (HUMARA) assay is the newest of such methods, and the majority of the female population can be assessed by this relatively simple procedure. One problem in using these clonality analysis methods, however, is that there may be significant variation in Lyonization in blood cells in normal individuals. To determine the diversity in X-chromosome methylation patterns, which reflect Lyonization, assessed by the HUMARA assay in the supposedly normal population, we analyzed granulocytes and T cells from 97 relatively young (18- to 35-year-old) healthy female volunteers. We found that the methylation patterns in the two HUMARA alleles were distributed even more widely, both in granuloctyes and in T cells, than previously reported with other methods. We also found that the deviation of methylation in granulocytes and T cells was well correlated. Thus, we conclude that appropriate controls from the same individuals, such as T cells in the case of stem cell disorders, should always be employed to conclusively determine whether certain cells of hematopoietic origin are clonal.",
author = "Yousuke Nakahara and Hitoshi Suzuki and Haruhiko Ohashi and Sonoko Hatano and Akihiro Tomita and Tomohiro Kinoshita and Takashi Murate and Hidehiko Saito and Tomomitsu Hotta",
year = "1999",
month = "12",
day = "1",
language = "English",
volume = "69",
pages = "237--243",
journal = "International Journal of Hematology",
issn = "0925-5710",
publisher = "Springer Japan",
number = "4",

}

Nakahara, Y, Suzuki, H, Ohashi, H, Hatano, S, Tomita, A, Kinoshita, T, Murate, T, Saito, H & Hotta, T 1999, 'Clonality analysis of granulocytes and T lymphocytes in healthy females by the PCR-based HUMARA method', International Journal of Hematology, 巻. 69, 番号 4, pp. 237-243.

Clonality analysis of granulocytes and T lymphocytes in healthy females by the PCR-based HUMARA method. / Nakahara, Yousuke; Suzuki, Hitoshi; Ohashi, Haruhiko; Hatano, Sonoko; Tomita, Akihiro; Kinoshita, Tomohiro; Murate, Takashi; Saito, Hidehiko; Hotta, Tomomitsu.

:: International Journal of Hematology, 巻 69, 番号 4, 01.12.1999, p. 237-243.

研究成果: Article

TY - JOUR

T1 - Clonality analysis of granulocytes and T lymphocytes in healthy females by the PCR-based HUMARA method

AU - Nakahara, Yousuke

AU - Suzuki, Hitoshi

AU - Ohashi, Haruhiko

AU - Hatano, Sonoko

AU - Tomita, Akihiro

AU - Kinoshita, Tomohiro

AU - Murate, Takashi

AU - Saito, Hidehiko

AU - Hotta, Tomomitsu

PY - 1999/12/1

Y1 - 1999/12/1

N2 - Clonality analysis utilizing X-chromosome inactivation has been used in the study of various diseases, including hematological malignancies. The human androgen receptor gene (HUMARA) assay is the newest of such methods, and the majority of the female population can be assessed by this relatively simple procedure. One problem in using these clonality analysis methods, however, is that there may be significant variation in Lyonization in blood cells in normal individuals. To determine the diversity in X-chromosome methylation patterns, which reflect Lyonization, assessed by the HUMARA assay in the supposedly normal population, we analyzed granulocytes and T cells from 97 relatively young (18- to 35-year-old) healthy female volunteers. We found that the methylation patterns in the two HUMARA alleles were distributed even more widely, both in granuloctyes and in T cells, than previously reported with other methods. We also found that the deviation of methylation in granulocytes and T cells was well correlated. Thus, we conclude that appropriate controls from the same individuals, such as T cells in the case of stem cell disorders, should always be employed to conclusively determine whether certain cells of hematopoietic origin are clonal.

AB - Clonality analysis utilizing X-chromosome inactivation has been used in the study of various diseases, including hematological malignancies. The human androgen receptor gene (HUMARA) assay is the newest of such methods, and the majority of the female population can be assessed by this relatively simple procedure. One problem in using these clonality analysis methods, however, is that there may be significant variation in Lyonization in blood cells in normal individuals. To determine the diversity in X-chromosome methylation patterns, which reflect Lyonization, assessed by the HUMARA assay in the supposedly normal population, we analyzed granulocytes and T cells from 97 relatively young (18- to 35-year-old) healthy female volunteers. We found that the methylation patterns in the two HUMARA alleles were distributed even more widely, both in granuloctyes and in T cells, than previously reported with other methods. We also found that the deviation of methylation in granulocytes and T cells was well correlated. Thus, we conclude that appropriate controls from the same individuals, such as T cells in the case of stem cell disorders, should always be employed to conclusively determine whether certain cells of hematopoietic origin are clonal.

UR - http://www.scopus.com/inward/record.url?scp=0033142606&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033142606&partnerID=8YFLogxK

M3 - Article

C2 - 10407580

AN - SCOPUS:0033142606

VL - 69

SP - 237

EP - 243

JO - International Journal of Hematology

JF - International Journal of Hematology

SN - 0925-5710

IS - 4

ER -