TY - JOUR
T1 - Cloning and sequencing of a novel human gene that encodes a putative target protein of Nesh-SH3
AU - Matsuda, S.
AU - Iriyama, C.
AU - Yokozaki, S.
AU - Ichigotani, Y.
AU - Shirafuji, N.
AU - Yamaki, K.
AU - Hayakawa, T.
AU - Hamaguchi, M.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2001
Y1 - 2001
N2 - By using a conventional two-hybrid technique with an Src homology 3 (SH3) domain of Nesh as the bait protein, a novel full-length cDNA was isolated and sequenced from a human placenta cDNA library. This cDNA consists of 3023 bp and has a predicted open reading frame that encodes 486 amino acids. It possesses an SH3 binding motif, a nuclear targeting sequence, and no catalytic domain. Overall, it has no similarity to known molecules involved in a signaling cascade. Polymerase Chair reaction-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels localized the gene on human chromosome 3q12 near the marker D3S1271.
AB - By using a conventional two-hybrid technique with an Src homology 3 (SH3) domain of Nesh as the bait protein, a novel full-length cDNA was isolated and sequenced from a human placenta cDNA library. This cDNA consists of 3023 bp and has a predicted open reading frame that encodes 486 amino acids. It possesses an SH3 binding motif, a nuclear targeting sequence, and no catalytic domain. Overall, it has no similarity to known molecules involved in a signaling cascade. Polymerase Chair reaction-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels localized the gene on human chromosome 3q12 near the marker D3S1271.
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U2 - 10.1007/s100380170049
DO - 10.1007/s100380170049
M3 - Article
C2 - 11501947
AN - SCOPUS:0034879525
SN - 1434-5161
VL - 46
SP - 483
EP - 486
JO - Journal of Human Genetics
JF - Journal of Human Genetics
IS - 8
ER -