抄録
We performed pre-embedding electron microscopic study for visualizing the antigen and genome of severe fever with thrombocytopenia syndrome (SFTS) virus in the cytoplasm of macrophages of the human splenic red pulp, both requesting preheating treatment of sections. To pursue this, coated glass slides with unique characteristics are needed. Namely, during staining they must prevent detaching off sections, but after staining the sections must be transferred to epoxy resin. Aminopropyltriexoxysilane-coated glass slides, widely used for immunostaining, were resistant to transfer to epoxy resin. In contrast, coated glass slides designated as Thinlayer Advanced Cytology Assay System (TACAS) were suitable for this purpose. The technique is also applicable to the coated glass slide-requiring cytology practice, in which immunocytochemical evaluation is needed after cell transfer to another glass slide.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 153-157 |
| ページ数 | 5 |
| ジャーナル | Acta Histochemica et Cytochemica |
| 巻 | 48 |
| 発行部数 | 5 |
| DOI |
|
| 出版物ステータス | Published - 29-10-2015 |
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All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
- Biochemistry
- Physiology
- Histology
- Cell Biology
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Coated glass slides TACAS are applicable to heat-assisted immunostaining and In situ hybridization at the electron microscopy level. / Matsui, Takahiro; Onouchi, Takanori; Shiogama, Kazuya; Mizutani, Yasuyoshi; Inada, Ken Ichi; Yu, Fuxun; Hayasaka, Daisuke; Morita, Koichi; Ogawa, Hirohisa; Mahara, Fumihiko; Tsutsumi, Yutaka.
：: Acta Histochemica et Cytochemica, 巻 48, 番号 5, 29.10.2015, p. 153-157.研究成果: Comment/debate
TY - JOUR
T1 - Coated glass slides TACAS are applicable to heat-assisted immunostaining and In situ hybridization at the electron microscopy level
AU - Matsui, Takahiro
AU - Onouchi, Takanori
AU - Shiogama, Kazuya
AU - Mizutani, Yasuyoshi
AU - Inada, Ken Ichi
AU - Yu, Fuxun
AU - Hayasaka, Daisuke
AU - Morita, Koichi
AU - Ogawa, Hirohisa
AU - Mahara, Fumihiko
AU - Tsutsumi, Yutaka
PY - 2015/10/29
Y1 - 2015/10/29
N2 - We performed pre-embedding electron microscopic study for visualizing the antigen and genome of severe fever with thrombocytopenia syndrome (SFTS) virus in the cytoplasm of macrophages of the human splenic red pulp, both requesting preheating treatment of sections. To pursue this, coated glass slides with unique characteristics are needed. Namely, during staining they must prevent detaching off sections, but after staining the sections must be transferred to epoxy resin. Aminopropyltriexoxysilane-coated glass slides, widely used for immunostaining, were resistant to transfer to epoxy resin. In contrast, coated glass slides designated as Thinlayer Advanced Cytology Assay System (TACAS) were suitable for this purpose. The technique is also applicable to the coated glass slide-requiring cytology practice, in which immunocytochemical evaluation is needed after cell transfer to another glass slide.
AB - We performed pre-embedding electron microscopic study for visualizing the antigen and genome of severe fever with thrombocytopenia syndrome (SFTS) virus in the cytoplasm of macrophages of the human splenic red pulp, both requesting preheating treatment of sections. To pursue this, coated glass slides with unique characteristics are needed. Namely, during staining they must prevent detaching off sections, but after staining the sections must be transferred to epoxy resin. Aminopropyltriexoxysilane-coated glass slides, widely used for immunostaining, were resistant to transfer to epoxy resin. In contrast, coated glass slides designated as Thinlayer Advanced Cytology Assay System (TACAS) were suitable for this purpose. The technique is also applicable to the coated glass slide-requiring cytology practice, in which immunocytochemical evaluation is needed after cell transfer to another glass slide.
UR - http://www.scopus.com/inward/record.url?scp=84945955740&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84945955740&partnerID=8YFLogxK
U2 - 10.1267/ahc.15012
DO - 10.1267/ahc.15012
M3 - Comment/debate
VL - 48
SP - 153
EP - 157
JO - Acta Histochemica et Cytochemica
JF - Acta Histochemica et Cytochemica
SN - 0044-5991
IS - 5
ER -