Colistin Resistance in Carbapenem-Resistant Klebsiella pneumoniae: Laboratory Detection and Impact on Mortality

Laura J. Rojas, Madiha Salim, Eric Cober, Sandra S. Richter, Federico Perez, Robert A. Salata, Robert C. Kalayjian, Richard R. Watkins, Steve Marshall, Susan D. Rudin, T. Nicholas Domitrovic, Andrea M. Hujer, Kristine M. Hujer, Yohei Doi, Keith S. Kaye, Scott Evans, Vance G. Fowler, Robert A. Bonomo, David Van Duin

研究成果: ジャーナルへの寄稿学術論文査読

171 被引用数 (Scopus)

抄録

Background.: Polymyxins including colistin are an important "last-line"treatment for infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKp). Increasing use of colistin has led to resistance to this cationic antimicrobial peptide. Methods.: A cohort nested within the Consortium on Resistance against Carbapenems in Klebsiella pneumoniae (CRACKLE) was constructed of patients with infection, or colonization with CRKp isolates tested for colistin susceptibility during the study period of December, 2011 to October, 2014. Reference colistin resistance determination as performed by broth macrodilution was compared to results from clinical microbiology laboratories (Etest) and to polymyxin resistance testing. Each patient was included once, at the time of their first colistin-tested CRKp positive culture. Time to 30-day in-hospital all-cause mortality was evaluated by Kaplan-Meier curves and Cox proportional hazard modeling. Results.: In 246 patients with CRKp, 13% possessed ColR CRKp. ColR was underestimated by Etest (very major error rate = 35%, major error rate = 0.4%). A variety of rep-PCR strain types were encountered in both the ColS and the ColR groups. Carbapenem resistance was mediated primarily by blaKPC-2 (46%) and blaKPC-3 (50%). ColR was associated with increased hazard for in-hospital mortality (aHR 3.48; 95% confidence interval, 1.73-6.57; P <. 001). The plasmid-associated ColR genes, mcr-1 and mcr-2 were not detected in any of the ColR CRKp. Conclusions.: In this cohort, 13% of patients with CRKp presented with ColR CRKp. The apparent polyclonal nature of the isolates suggests de novo emergence of ColR in this cohort as the primary factor driving ColR. Importantly, mortality was increased in patients with ColR isolates.

本文言語英語
ページ(範囲)711-718
ページ数8
ジャーナルClinical Infectious Diseases
64
6
DOI
出版ステータス出版済み - 15-03-2017
外部発表はい

All Science Journal Classification (ASJC) codes

  • 微生物学(医療)
  • 感染症

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