Collagen sponge scaffolds containing growth factors for the functional regeneration of tracheal epithelium

Ryosuke Nakamura, Tatsuya Katsuno, Morimasa Kitamura, Masaru Yamashita, Takuya Tsuji, Ryo Suzuki, Yo Kishimoto, Atsushi Suehiro, Ichiro Tateya, Tatsuo Nakamura, Koichi Omori

研究成果: ジャーナルへの寄稿学術論文査読

17 被引用数 (Scopus)

抄録

Tracheal epithelia have barrier and mucociliary clearance functions that prevent invasion of extraneous particles and infectious materials. Hence, following tracheal reconstructions, functional and morphological regeneration of epithelia is required to prevent respiratory declines and infectious diseases. Although growth factors (GFs) promote the regeneration of tracheal epithelial morphologies, it remains unclear whether tracheal grafts containing GFs are beneficial for regeneration of tracheal epithelial functions. Thus, we fabricated collagen sponge scaffolds containing insulin-like GF-1 (IGF-1) and the basic fibroblast, hepatocyte, and epidermal GFs (bFGFs, HGFs, and EGFs, respectively), and we evaluated the effects of the grafts on the functional regeneration of tracheal epithelia. Partial tracheal defects were imposed surgically, and collagen sponges containing IGF-1, bFGF, HGF, or EGF were then transplanted to defect sites. Subsequent immunofluorescence studies suggested that EGF and bFGF contribute to regular distributions of tight junction molecules, and tracer permeability assays suggested that EGF and bFGF promote regeneration of barrier function. Increased ciliogenesis was also observed using scanning electron microscopy in reconstructed regions treated with EGF- and bFGF-supplemented collagen sponges. However, bFGF-supplemented collagen sponges led to greater microsphere transport than did EGF-supplemented sponges. The present data suggested that collagen sponge scaffold containing bFGF promotes functional regeneration of tracheal epithelial tissues.

本文言語英語
ページ(範囲)835-845
ページ数11
ジャーナルJournal of Tissue Engineering and Regenerative Medicine
13
5
DOI
出版ステータス出版済み - 05-2019
外部発表はい

All Science Journal Classification (ASJC) codes

  • 医学(その他)
  • 生体材料
  • 生体医工学

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