Comparative studies of the same adenocarcinoma cells, macrophages, and mesothelial cells by light microscopy, scanning electron microscopy, and transmission electron microscopy

Chiyuki Kaneko, Hajime Niimi, Masanori Shinzato, Mikihiro Shamoto

研究成果: Article査読

12 被引用数 (Scopus)

抄録

The identification of cells in body cavities of cancer patients is sometimes difficult to make. In order to make a definite cytological diagnosis, we observed the same cells by using light microscopy (LM)‐scanning electron microscopy (SEM)‐transmission electron microscopy (TEM). In this study we first stained cells by the Papanicolaou method after fixation in 1% glutaraldehyde for LM, and then attempted to observe them successively by SEM‐TEM after fixation in 1% paraformaldehyde and 1.25% Os04. Our method and procedures in examining successively one and the same cells in body cavity fluids by using LM, SEM, and TEM ensured accurate discrimination among adenocarcinoma cells, mesothelial cells, and macrophages. The results of this study suggest that LM‐SEM‐TEM may be of diagnostic value in distinguishing among mesothelial cells, macrophages, and adenocarcinoma cells. This method also succeeded in disclosing differences between the ultrastructure of the cell surfaces, and those of the cytoplasm, and of the nuclei It is desirable that LM‐SEM‐TEM observation can be introduced into various aspects in order to obtain an improvement in the diagnosis by cytologic examination, the judgment of therapeutical effects, drug selection, and prognostic presumption. Diagn Cytopathol 1994; 11:333–342. © 1994 Wiley‐Liss, Inc.

本文言語English
ページ(範囲)333-342
ページ数10
ジャーナルDiagnostic Cytopathology
11
4
DOI
出版ステータスPublished - 12-1994

All Science Journal Classification (ASJC) codes

  • 病理学および法医学
  • 組織学

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