TY - JOUR
T1 - Comparison of phospholipid N-methylation activity in rat submandibular salivary gland and liver
AU - Yashiro, K.
AU - Kameyama, Y.
AU - Mizuno, M.
AU - Okada, A.
AU - Yokota, Y.
N1 - Funding Information:
Acknowledgements-We are thankful to MS Teruyo Tanahashi for her assistance. This study was supported in part by a grant from Miyata Science Research Foundation of Asahi University and Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan.
PY - 1989
Y1 - 1989
N2 - Successive phospholipid N-methylation from phosphatidylcholine to phosphatidylethanolamine in submandibular gland and liver microsomes proceeded without the addition of exogenous phospholipid substrate. Methylation activity in the submandibular microsomes showed different susceptibilities to various detergents than the liver enzyme and also partially required Mg2+. However, the three methylation steps could not be distinguished by their Mg2+ requirements. Ca2+ had no effect on the activity. The methylation activity in submandibular gland was much lower than in liver. Chronic administration of isoproterenol, which causes an increase of phosphatidylcholine in membrane phospholipids of salivary glands, decreased methylation activity in the submandibular gland. Thus the increase in phosphatidylcholine in isoproterenol-treated rat salivary glands may not be derived from the phospholipid methylation pathway, but may be due to stimulation of other routes of phosphatidylcholine metabolism.
AB - Successive phospholipid N-methylation from phosphatidylcholine to phosphatidylethanolamine in submandibular gland and liver microsomes proceeded without the addition of exogenous phospholipid substrate. Methylation activity in the submandibular microsomes showed different susceptibilities to various detergents than the liver enzyme and also partially required Mg2+. However, the three methylation steps could not be distinguished by their Mg2+ requirements. Ca2+ had no effect on the activity. The methylation activity in submandibular gland was much lower than in liver. Chronic administration of isoproterenol, which causes an increase of phosphatidylcholine in membrane phospholipids of salivary glands, decreased methylation activity in the submandibular gland. Thus the increase in phosphatidylcholine in isoproterenol-treated rat salivary glands may not be derived from the phospholipid methylation pathway, but may be due to stimulation of other routes of phosphatidylcholine metabolism.
UR - https://www.scopus.com/pages/publications/0024377506
UR - https://www.scopus.com/pages/publications/0024377506#tab=citedBy
U2 - 10.1016/0003-9969(89)90009-5
DO - 10.1016/0003-9969(89)90009-5
M3 - Article
C2 - 2818269
AN - SCOPUS:0024377506
SN - 0003-9969
VL - 34
SP - 203
EP - 208
JO - Archives of Oral Biology
JF - Archives of Oral Biology
IS - 3
ER -