Comparison of strategies for the construction of libraries of artificial antibodies

Construction of libraries of artificial antibodies has been reported by several groups of investigators. Various forms of antibody fused to a surface protein, cpIII, are expressed on the surface of filamentous phage. Since phages that encode desired antibodies can be easily grown in Escherichia coli after selection with target antigens, the phage-display antibody system appears to be very useful for various biological purposes. In this brief review, recent progress in research into the production of artificial antibodies is summarized and the strategies used for the construction of libraries of artificial antibodies are compared.