Comparison of the promoter regions of H-2K and H-2Kbm1 class I MHC genes

Kazuo Ozawa, Fujiko Saka, Issay Kitabayashi, Takashi Lmai, Eiichi Soeda, Alain Israel, Gabriel Gachelin, Kazushige Yokoyama

研究成果: ジャーナルへの寄稿学術論文査読

5 被引用数 (Scopus)

抄録

The 2.0 kb-long nucleotide sequences of the promoter regions of two closely related class I genes of the mouse major histocompatibility complex (H-2Kb and H-2Kbm1) have been determined and compared. The promoter sequence of the H-2Kbm1 gene differs from that of the H-2Kb gene by a single deletion of a 'C' at position -456 in the upstream region of H-2Kbm1 gene. The actual existence of this deletion of a single base in genomic DNA has been verified by genomic DNA hybridization, using oligonucleotide probes specific for H-2Kbm1 or H-2Kb respectively. The effect on the enhancer activity of H-2Kbm1 promoter region of the difference at position -456 has been analyzed by the chloramphenicol acetyltransferase (CAT) assay, using appropriate Ddel fragments (-533 to -408 for H-2Kbm1; - 534 to - 408 for H-2Kb) cloned downstream of pH-2(367)CAT gene construct. The CAT activity determined by the H-2Kbm1 fragment was about 3-fold higher than that of H-2Kb, a result which probably accounts for the higher level of the H-2Kbm1 transcript and antigen in lymph node cells.

本文言語英語
ページ(範囲)4185-4190
ページ数6
ジャーナルNucleic acids research
18
14
DOI
出版ステータス出版済み - 25-07-1990
外部発表はい

All Science Journal Classification (ASJC) codes

  • 遺伝学

フィンガープリント

「Comparison of the promoter regions of H-2K and H-2Kbm1 class I MHC genes」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル