Comparison of the tube test and column agglutination techniques for anti-A/-B antibody titration in healthy individuals

Hideaki Matsuura, Yoshiki Akatsuka, Takahiro Matsuno, Yukari Sugiura, Shoko Arakawa, Shota Oikawa, Jumpei Yoshida, Mitsuaki Kosugi, Nobuhiko Emi

研究成果: Article

1 引用 (Scopus)

抜粋

Background and Objectives: Determination of the anti-A/-B titre pre- and post-transplantation is beneficial for treatment selection. Currently, the recommended method for antibody titration is the tube test (TT) assay. Dithiothreitol (DTT) is used for IgM antibody inactivation. Recently, a fully automated antibody titration assay using the column agglutination technique (CAT) was developed (auto-CAT). Our aim was to compare the auto-CAT and TT techniques for ABO antibody titration, to evaluate the effectiveness of DTT-treated plasma for use with auto-CAT and to define the cut-off value for antibody titration by auto-CAT. Materials and Methods: We enrolled 30 healthy individuals, including 10 each for blood types A, B and O. We performed antibody titre measurement using the TT technique and auto-CAT simultaneously. Auto-CAT uses the bead column agglutination technology. Results: With the auto-CAT cut-off value set to weak (w)+ with DTT treatment plasma, the concordance rate was 45%, and the weighted kappa value between TT and auto-CAT results was 0·994 in all subjects. Furthermore, there was a significant positive correlation between the anti-A/-B titre results obtained using the TT technique and auto-CAT in all blood types. Moreover, a positive bias (falsely elevated end-points due to agglomeration of A/B cells) was not observed in auto-CAT testing using DTT-treated plasma. Conclusion: Our results show that 1+ agglutination using the TT technique is equivalent to w+ agglutination obtained using auto-CAT. We recommend that DTT may be used with auto-CAT to measure antibody titres. Thus, we suggest that auto-CAT is useful for antibody titration in routine examination.

元の言語English
ページ(範囲)787-794
ページ数8
ジャーナルVox Sanguinis
113
発行部数8
DOI
出版物ステータスPublished - 11-2018

All Science Journal Classification (ASJC) codes

  • Hematology

フィンガープリント Comparison of the tube test and column agglutination techniques for anti-A/-B antibody titration in healthy individuals' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Matsuura, H., Akatsuka, Y., Matsuno, T., Sugiura, Y., Arakawa, S., Oikawa, S., Yoshida, J., Kosugi, M., & Emi, N. (2018). Comparison of the tube test and column agglutination techniques for anti-A/-B antibody titration in healthy individuals. Vox Sanguinis, 113(8), 787-794. https://doi.org/10.1111/vox.12713