Comparisons of cochleotoxicity among three gentamicin compounds following intratympanic application

Masamichi Kobayashi, Michihiko Sone, Masayuki Umemura, Toshitaka Nabeshima, Tsutomu Nakashima, Sten Hellström

研究成果: Article

9 引用 (Scopus)

抄録

Conclusion. Among the three main gentamicin (GM) compounds following intratympanic application, the cochleotoxicity of C2 was the most severe, whereas that of C1a was the weakest. Understanding of the different cochleotoxicity characteristics of each compound may be of use in future custom-made intratympanic therapy for Ménière's disease. Objective. To investigate differences in cochleotoxicity among three major GM compounds following intratympanic application. Materials and methods. Three GM compounds (C1, C2, and C1a) were isolated. Sprague-Dawley rats were treated every 2 days for 2 weeks with intratympanic application of saline, GM complex, C1, C2, and C1a. The cochleotoxicity of each compound was assessed by measuring auditory brainstem response (ABR) and through morphological analyses using scanning electron microscopy. Results. The ABR threshold of the C2 group was found to be more impaired than those of the other groups. The C1a group showed the mildest elevation of the ABR thresholds. Morphological analyses revealed that the proportion of remaining outer hair cells (OHCs) was the lowest in animals treated with C2. Morphologically, the C1 and C1a groups showed the least damage to OHCs.

元の言語English
ページ(範囲)245-249
ページ数5
ジャーナルActa Oto-Laryngologica
128
発行部数3
DOI
出版物ステータスPublished - 22-02-2008
外部発表Yes

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Gentamicins
Outer Auditory Hair Cells
Brain Stem Auditory Evoked Potentials
Electron Scanning Microscopy
Sprague Dawley Rats
gentamicin C
Therapeutics

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

これを引用

Kobayashi, Masamichi ; Sone, Michihiko ; Umemura, Masayuki ; Nabeshima, Toshitaka ; Nakashima, Tsutomu ; Hellström, Sten. / Comparisons of cochleotoxicity among three gentamicin compounds following intratympanic application. :: Acta Oto-Laryngologica. 2008 ; 巻 128, 番号 3. pp. 245-249.
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Comparisons of cochleotoxicity among three gentamicin compounds following intratympanic application. / Kobayashi, Masamichi; Sone, Michihiko; Umemura, Masayuki; Nabeshima, Toshitaka; Nakashima, Tsutomu; Hellström, Sten.

:: Acta Oto-Laryngologica, 巻 128, 番号 3, 22.02.2008, p. 245-249.

研究成果: Article

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N2 - Conclusion. Among the three main gentamicin (GM) compounds following intratympanic application, the cochleotoxicity of C2 was the most severe, whereas that of C1a was the weakest. Understanding of the different cochleotoxicity characteristics of each compound may be of use in future custom-made intratympanic therapy for Ménière's disease. Objective. To investigate differences in cochleotoxicity among three major GM compounds following intratympanic application. Materials and methods. Three GM compounds (C1, C2, and C1a) were isolated. Sprague-Dawley rats were treated every 2 days for 2 weeks with intratympanic application of saline, GM complex, C1, C2, and C1a. The cochleotoxicity of each compound was assessed by measuring auditory brainstem response (ABR) and through morphological analyses using scanning electron microscopy. Results. The ABR threshold of the C2 group was found to be more impaired than those of the other groups. The C1a group showed the mildest elevation of the ABR thresholds. Morphological analyses revealed that the proportion of remaining outer hair cells (OHCs) was the lowest in animals treated with C2. Morphologically, the C1 and C1a groups showed the least damage to OHCs.

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