Conditional knockdown of target gene expression by tetracycline regulated transcription of double strand RNA

Xubin Hou, Minoru Omi, Hidekiyo Harada, Shunsuke Ishii, Yoshiko Takahashi, Harukazu Nakamura

研究成果: Article査読

17 被引用数 (Scopus)

抄録

In vivo electroporation has served as an effective tool for the study of developmental biology. Here we report tetracycline inducible gene knockdown by electroporation. Our system consists of genome integration of a cassette encoding long double strand RNA (dsRNA) of a gene of interest by electroporation, transcription of which is assured by RNA polymerase II, and induction of transcription of dsRNA by tetracyclin. Long dsRNA decapped by ribozyme in the cassette and without poly A tail is processed into siRNA within nuclei. We could successfully induce knockdown of En2 and Coactosin by Dox administration.

本文言語English
ページ(範囲)69-75
ページ数7
ジャーナルDevelopment Growth and Differentiation
53
1
DOI
出版ステータスPublished - 01-2011
外部発表はい

All Science Journal Classification (ASJC) codes

  • 発生生物学
  • 細胞生物学

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