TY - JOUR
T1 - Construction and characterization of an infectious molecular clone derived from the CRF01_AE primary isolate of HIV type 1
AU - Mukai, Tetsu
AU - Komoto, Satoshi
AU - Kurosu, Takeshi
AU - Palacios, J. Alejandro
AU - Yong-Gang, L. I.
AU - Auwanit, Wattana
AU - Tatsumi, Masashi
AU - Ikuta, Kazuyoshi
PY - 2002
Y1 - 2002
N2 - An infectious molecular clone (named p95TNIH022) was constructed using long-range polymerase chain reaction products derived from a clinical isolate (95TNIH022) of HIV-1 CRF01_AE obtained from an asymptomatic Thai carrier in 1995. The virus in the supernatant from p95TNIH022-transfected 293T cells showed infectivity in peripheral blood mononuclear cells (PBMCs) as well as in MAGIC5 cells, which express CD4 and CCR5, but not in the original MAGI cells, indicating that p95TNIH022 is an infectious molecular clone with CCR5 tropism. Interestingly, p95TNIH022-derived virus induced profound cell killing in infected PBMCs, as in cells infected with the parental isolate.
AB - An infectious molecular clone (named p95TNIH022) was constructed using long-range polymerase chain reaction products derived from a clinical isolate (95TNIH022) of HIV-1 CRF01_AE obtained from an asymptomatic Thai carrier in 1995. The virus in the supernatant from p95TNIH022-transfected 293T cells showed infectivity in peripheral blood mononuclear cells (PBMCs) as well as in MAGIC5 cells, which express CD4 and CCR5, but not in the original MAGI cells, indicating that p95TNIH022 is an infectious molecular clone with CCR5 tropism. Interestingly, p95TNIH022-derived virus induced profound cell killing in infected PBMCs, as in cells infected with the parental isolate.
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U2 - 10.1089/088922202753747932
DO - 10.1089/088922202753747932
M3 - Article
C2 - 12036488
AN - SCOPUS:0036266794
SN - 0889-2229
VL - 18
SP - 585
EP - 589
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
IS - 8
ER -
