Construction of radiation-reduced hybrids and their use in mapping of microclones from chromosome 10p11.2-q11.2

Shoichi Fujita, Eisei Shin, Tsutomu Nakamura, Hiroki Kurahashi, Yasufumi Kaneda, Kiyoji Tanaka, Takesada Mori, Shin ichiro Takai, Isamu Nishisho

研究成果: ジャーナルへの寄稿学術論文査読

1 被引用数 (Scopus)

抄録

Radiation-reduced hybrids for mapping of DNA markers in the pericentromeric region of chromosome 10 were developed. A Chinese hamster/human somatic cell hybrid (762-8A) carrying chromosomes 10 and Y as the only human material were exposed to 40,000 rads of irradiation and then rescued by fusion with non-irradiated recipient Chinese hamster cells (GM459). Southern hybridization analyses revealed that 10 of 128 HAT-resistant clones contained human chromosomal fragments corresponding to at least one marker locus between FNRB (10p-11.2) and RBP3 (10q11.2). These hybrids were then used to map microdissection clones previously isolated and roughly mapped to this chromosomal region by fluorescence in situ hybridization (FISH). Two of the six microclones studied could be mapped to the proximity of the D10-S102 locus. These radiation hybrids are useful for the construction of refined genetic maps of the pericentromeric region of chromosome 10.

本文言語英語
ページ(範囲)361-370
ページ数10
ジャーナルThe Japanese Journal of Human Genetics
38
4
DOI
出版ステータス出版済み - 12-1993
外部発表はい

All Science Journal Classification (ASJC) codes

  • 遺伝学(臨床)

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