Core Transcription Factors Promote Induction of PAX3-Positive Skeletal Muscle Stem Cells

Takahiko Sato, Koki Higashioka, Hidetoshi Sakurai, Takuya Yamamoto, N. Goshima, M. Ueno, Chie Sotozono

研究成果: ジャーナルへの寄稿学術論文査読

17 被引用数 (Scopus)

抄録

The use of adult skeletal muscle stem cells (MuSCs) for cell therapy has been attempted for decades, but still encounters considerable difficulties. MuSCs derived from human induced pluripotent stem cells (hiPSCs) are promising candidates for stem cell therapy to treat Duchenne muscular dystrophy (DMD). Here we report that four transcription factors, HEYL, KLF4, MYOD, and PAX3, selected by comprehensive screening of different MuSC populations, enhance the derivation of PAX3-positive myogenic progenitors from fibroblasts and hiPSCs, using medium that promotes the formation of presomitic mesoderm. These induced PAX3-positive cells contribute efficiently to the repair of DMD-damaged myofibers and also reconstitute the MuSC population. These studies demonstrate how a combination of core transcription factors can fine-tune the derivation of MuSCs capable of contributing to the repair of adult skeletal muscle. In this article, Sato and colleagues show that four transcription factors, Heyl, Klf4, MyoD, and Pax3, selected by comprehensive screening of mouse muscle stem cells, enhance the derivation of PAX3-positive myogenic progenitors from fibroblasts and hiPSCs. These induced PAX3-positive cells contribute efficiently to the repair of DMD-damaged myofibers and also reconstitute stem cell population.

本文言語英語
ページ(範囲)352-365
ページ数14
ジャーナルStem Cell Reports
13
2
DOI
出版ステータス出版済み - 13-08-2019

All Science Journal Classification (ASJC) codes

  • 生化学
  • 遺伝学
  • 発生生物学
  • 細胞生物学

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