Corticotropin-releasing factor receptor antagonist attenuates LPS-induced increase of GTP cyclohydrolase I expression at murine locus coeruleus

Yoko S. Kaneko, Keiji Mori, Akira Nakashima, Ikuko Nagatsu, Toshiharu Nagatsu, Akira Ota

研究成果: Article

1 引用 (Scopus)

抜粋

It has been reported that corticotropin-releasing factor (CRF) is involved in the regulation of norepinephrine neuron responses to stress such as an immobilized stress. Furthermore, systemic lipopolysaccharide (LPS) injection upregulates the transcription of the genes encoding CRF and CRF type 1 receptor in the paraventricular nucleus of the hypothalamus. We have already reported that an increase in norepinephrine turnover within the murine locus coeruleus is accompanied by septic shock triggered by LPS intraperitoneal injection. We also elucidated that the expression levels of the enzymes involved in the catecholamine biosynthesis were altered by peripheral LPS injection. Collectively, the effects of CRF on the expression levels of the enzymes at murine locus coeruleus were investigated by peripherally injecting CP-154,526, a CRF receptor type 1 antagonist. Pretreatment with CP-154,526 attenuated the increase in expression levels of GTP cyclohydrolase I mRNA due to intraperitoneal LPS injection at 4 h after the injection. However, no effects on the expression level of tyrosine hydroxylase mRNA at the site were observed. Taken together with the fact that LPS injection enhances tetrahydrobiopterin biosynthesis at locus coeruleus, CP-154,526 may attenuate the increase of NE turnover by suppressing the enhanced GCH expression level at the site caused by peripheral LPS injection.

元の言語English
ページ(範囲)289-297
ページ数9
ジャーナルBiogenic Amines
19
発行部数4-6
DOI
出版物ステータスPublished - 01-12-2005

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Pharmacology

フィンガープリント Corticotropin-releasing factor receptor antagonist attenuates LPS-induced increase of GTP cyclohydrolase I expression at murine locus coeruleus' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用