Cripto-1 enhances the canonical Wnt/β-catenin signaling pathway by binding to LRP5 and LRP6 co-receptors

Tadahiro Nagaoka, Hideaki Karasawa, Thomas Turbyville, Maria Cristina Rangel, Nadia P. Castro, Monica Gonzales, Alyson Baker, Masaharu Seno, Stephen Lockett, Yoshimi E. Greer, Jeffrey S. Rubin, David S. Salomon, Caterina Bianco

研究成果: Article

33 引用 (Scopus)

抜粋

Cripto-1 is implicated in multiple cellular events, including cell proliferation, motility and angiogenesis, through the activation of an intricate network of signaling pathways. A crosstalk between Cripto-1 and the canonical Wnt/β-catenin signaling pathway has been previously described. In fact, Cripto-1 is a downstream target gene of the canonical Wnt/β-catenin signaling pathway in the embryo and in colon cancer cells and T-cell factor (Tcf)/lymphoid enhancer factor binding sites have been identified in the promoter and the first intronic region of the mouse and human Cripto-1 genes. We now demonstrate that Cripto-1 modulates signaling through the canonical Wnt/β-catenin/Tcf pathway by binding to the Wnt co-receptors low-density lipoprotein receptor-related protein (LRP) 5 and LRP6, which facilitates Wnt3a binding to LRP5 and LRP6. Cripto-1 functionally enhances Wnt3a signaling through cytoplasmic stabilization of β-catenin and elevated β-catenin/Tcf transcriptional activation. Conversely, Wnt3a further increases Cripto-1 stimulation of migration, invasion and colony formation in soft agar of HC11 mouse mammary epithelial cells, indicating that Cripto-1 and the canonical Wnt/β-catenin signaling co-operate in regulating motility and in vitro transformation of mammary epithelial cells.

元の言語English
ページ(範囲)178-189
ページ数12
ジャーナルCellular Signalling
25
発行部数1
DOI
出版物ステータスPublished - 01-01-2013
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Cell Biology

フィンガープリント Cripto-1 enhances the canonical Wnt/β-catenin signaling pathway by binding to LRP5 and LRP6 co-receptors' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Nagaoka, T., Karasawa, H., Turbyville, T., Rangel, M. C., Castro, N. P., Gonzales, M., Baker, A., Seno, M., Lockett, S., Greer, Y. E., Rubin, J. S., Salomon, D. S., & Bianco, C. (2013). Cripto-1 enhances the canonical Wnt/β-catenin signaling pathway by binding to LRP5 and LRP6 co-receptors. Cellular Signalling, 25(1), 178-189. https://doi.org/10.1016/j.cellsig.2012.09.024