TY - JOUR
T1 - Cytoplasmic Dynein Functions in Planar Polarization of Basal Bodies within Ciliated Cells
AU - Takagishi, Maki
AU - Esaki, Nobutoshi
AU - Takahashi, Kunihiko
AU - Takahashi, Masahide
N1 - Funding Information:
The ES cell line used for Daple knockout mice was generated by the KOMP (www.komp.org). We thank Shindo, A. (Nagoya University) for technical advice on cell imaging and Goshima, G. (Nagoya University) for technical advice and helpful discussions on microtubule dynamics. We would also like to thank Wallingford, J.B. (University of Texas at Austin) for helpful discussions. This work was supported by Grant-in-Aid for Scientific Research (S) 26221304 to M. Takahashi, Grant-in-Aid for Young Scientists (B) 17K15593 and Naito Foundation to M. Takagishi. Conceptualization, Methodology, Investigation, Writing, Project administration, Funding acquisition, and Supervision, M. Takagishi; Investigation and Resources, N.E.; Formal Analysis, K.T.; Funding acquisition, Resources, and Supervision, M. Takahashi. The authors declare no competing interests.
Funding Information:
The ES cell line used for Daple knockout mice was generated by the KOMP ( www.komp.org ). We thank Shindo, A. (Nagoya University) for technical advice on cell imaging and Goshima, G. (Nagoya University) for technical advice and helpful discussions on microtubule dynamics. We would also like to thank Wallingford, J.B. (University of Texas at Austin) for helpful discussions. This work was supported by Grant-in-Aid for Scientific Research (S) 26221304 to M. Takahashi, Grant-in-Aid for Young Scientists (B) 17K15593 and Naito Foundation to M. Takagishi.
Publisher Copyright:
© 2020 The Authors
PY - 2020/6/26
Y1 - 2020/6/26
N2 - Despite common consensus about the importance of planar cell polarity (PCP) proteins in tissue orientation, little is known about the mechanisms used by PCP proteins to promote planar polarization of cytoskeletons within individual cells. One PCP protein Fzd6 asymmetrically localizes to the apical cell membrane of multi-ciliated ependymal cells lining the lateral ventricular (LV) wall on the side that contacts cerebrospinal fluid flow. Individual ependymal cells have planar polarized microtubules that connect ciliary basal bodies (BBs) with the cell cortex of the Fzd side to coordinate cilia orientation. Here, we report that cytoplasmic dynein is anchored to the cell cortex of the Fzd side via an adapter protein Daple that regulates microtubule dynamics. Asymmetric localization of cortical dynein generates a pulling force on dynamic microtubules connected to BBs, which in turn orients BBs toward the Fzd side. This is required for coordinated cilia orientation on the LV wall.
AB - Despite common consensus about the importance of planar cell polarity (PCP) proteins in tissue orientation, little is known about the mechanisms used by PCP proteins to promote planar polarization of cytoskeletons within individual cells. One PCP protein Fzd6 asymmetrically localizes to the apical cell membrane of multi-ciliated ependymal cells lining the lateral ventricular (LV) wall on the side that contacts cerebrospinal fluid flow. Individual ependymal cells have planar polarized microtubules that connect ciliary basal bodies (BBs) with the cell cortex of the Fzd side to coordinate cilia orientation. Here, we report that cytoplasmic dynein is anchored to the cell cortex of the Fzd side via an adapter protein Daple that regulates microtubule dynamics. Asymmetric localization of cortical dynein generates a pulling force on dynamic microtubules connected to BBs, which in turn orients BBs toward the Fzd side. This is required for coordinated cilia orientation on the LV wall.
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U2 - 10.1016/j.isci.2020.101213
DO - 10.1016/j.isci.2020.101213
M3 - Article
AN - SCOPUS:85090165140
VL - 23
JO - iScience
JF - iScience
SN - 2589-0042
IS - 6
M1 - 101213
ER -