Cytoskeletal rearrangements and transcriptional activation of c-fos serum response element by Rho-kinase

Kazuyasu Chihara, Mutsuki Amano, Nao Nakamura, Takeo Yano, Masao Shibata, Toshiya Tokui, Hisashi Ichikawa, Reiko Ikebe, Mitsuo Ikebe, Kozo Kaibuchi

研究成果: Article査読

165 被引用数 (Scopus)

抄録

The small GTPase Rho is implicated in cytoskeletal rearrangements including stress fiber and focal adhesion formation and in the transcriptional activation of c-fos serum response element. In vitro, Rho- kinase, which is activated by Rho, phosphorylates not only myosin light chain (MLC) (thereby activating myosin ATPase) but also myosin phosphatase, thus inactivating myosin phosphatase. Rho-kinase is involved in the formation of stress fibers and focal adhesions in fibroblasts. Here we show that the expression of constitutively active Rho-kinase increased the level of MLC phosphorylation. The activity of Rho-kinase was necessary for maintaining the vinculin-containing focal adhesions, whereas organized actin stress fibers were not necessary for this. The microinjection of constitutively active Rho- kinase into fibroblasts induced the formation of focal adhesions to some extent under the conditions where organized actin stress fibers were disrupted. The expression of constitutively active Rho-kinase also stimulated the transcriptional activity of c-fos serum response element. These results suggest that Rho-kinase has distinct roles in divergent pathways downstream of Rho, which include MLC phosphorylation leading to stress fiber formation, focal adhesion formation, and gene expression.

本文言語English
ページ(範囲)25121-25127
ページ数7
ジャーナルJournal of Biological Chemistry
272
40
DOI
出版ステータスPublished - 03-10-1997
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

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