Cytotoxicity and glycan-binding properties of an 18 kDa lectin isolated from the marine sponge Halichondria okadai

Ryo Matsumoto, Yuki Fujii, Sarkar M.A. Kawsar, Robert A. Kanaly, Hidetaro Yasumitsu, Yasuhiro Koide, Imtiaj Hasan, Chihiro Iwahara, Yukiko Ogawa, Chang Hun Im, Shigeki Sugawara, Masahiro Hosono, Kazuo Nitta, Jiharu Hamako, Taei Matsui, Yasuhiro Ozeki

研究成果: Article査読

20 被引用数 (Scopus)

抄録

A divalent cation-independent lectin-HOL-18, with cytotoxic activity against leukemia cells, was purified from a demosponge, Halichondria okadai. HOL-18 is a 72 kDa tetrameric lectin that consists of four non-covalently bonded 18 kDa subunits. Hemagglutination activity of the lectin was strongly inhibited by chitotriose (GlcNAcβ1-4GlcNAcβ1-4GlcNAc), fetuin and mucins from porcine stomach and bovine submaxillary gland. Lectin activity was stable at pH 4-12 and temperatures lower than 60 °C. Frontal affinity chromatography with 16 types of pyridylaminated oligosaccharides indicated that the lectin had an affinity for N-linked complex-type and sphingolipid-type oligosaccharides with N-acetylated hexosamines and neuramic acid at the non-reducing termini. The lectin killed Jurkat leukemia T cells and K562 erythroleukemia cells in a dose- and carbohydrate-dependent manner.

本文言語English
ページ(範囲)323-338
ページ数16
ジャーナルToxins
4
5
DOI
出版ステータスPublished - 05-2012

All Science Journal Classification (ASJC) codes

  • 毒物学
  • 健康、毒物学および変異誘発

フィンガープリント

「Cytotoxicity and glycan-binding properties of an 18 kDa lectin isolated from the marine sponge Halichondria okadai」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル