抄録
D-Fructose dehydrogenase was immobilized behind a dialysis membrane on a carbon paste electrode containing p-benzoquinone. The electrode showed a current response to D-fructose due to the p-benzoquinone-mediated bioelectrocatalytic oxidation of D-fructose. The dependence of the current response on the potential applied to the electrode, pH of the solution, and temperature was studied. On this basis, measurements of D-fructose were done at 0.5 V vs. Ag/AgCl, pH 4.5 and 25°C using the fructose dehydrogenase-modified carbon paste electrode. D-Fructose in fruits was measured by this electrode method, in which the interference from L-ascorbic acid was taken into account. A method of elimination of the interference, which uses ascorbate oxidase, is also described.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 2919-2924 |
| ページ数 | 6 |
| ジャーナル | Agricultural and Biological Chemistry |
| 巻 | 54 |
| 発行部数 | 11 |
| DOI | |
| 出版物ステータス | Published - 11-1990 |
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All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)
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D-fructose dehydrogenase-modified carbon paste electrode containing p-benzoquinone as a mediated amperometric fructose sensor. / Ikeda, Tokuji; Matsushita, Fumio; Senda, Mitsugi.
：: Agricultural and Biological Chemistry, 巻 54, 番号 11, 11.1990, p. 2919-2924.研究成果: Article
TY - JOUR
T1 - D-fructose dehydrogenase-modified carbon paste electrode containing p-benzoquinone as a mediated amperometric fructose sensor
AU - Ikeda, Tokuji
AU - Matsushita, Fumio
AU - Senda, Mitsugi
PY - 1990/11
Y1 - 1990/11
N2 - D-Fructose dehydrogenase was immobilized behind a dialysis membrane on a carbon paste electrode containing p-benzoquinone. The electrode showed a current response to D-fructose due to the p-benzoquinone-mediated bioelectrocatalytic oxidation of D-fructose. The dependence of the current response on the potential applied to the electrode, pH of the solution, and temperature was studied. On this basis, measurements of D-fructose were done at 0.5 V vs. Ag/AgCl, pH 4.5 and 25°C using the fructose dehydrogenase-modified carbon paste electrode. D-Fructose in fruits was measured by this electrode method, in which the interference from L-ascorbic acid was taken into account. A method of elimination of the interference, which uses ascorbate oxidase, is also described.
AB - D-Fructose dehydrogenase was immobilized behind a dialysis membrane on a carbon paste electrode containing p-benzoquinone. The electrode showed a current response to D-fructose due to the p-benzoquinone-mediated bioelectrocatalytic oxidation of D-fructose. The dependence of the current response on the potential applied to the electrode, pH of the solution, and temperature was studied. On this basis, measurements of D-fructose were done at 0.5 V vs. Ag/AgCl, pH 4.5 and 25°C using the fructose dehydrogenase-modified carbon paste electrode. D-Fructose in fruits was measured by this electrode method, in which the interference from L-ascorbic acid was taken into account. A method of elimination of the interference, which uses ascorbate oxidase, is also described.
UR - http://www.scopus.com/inward/record.url?scp=0001448728&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0001448728&partnerID=8YFLogxK
U2 - 10.1080/00021369.1990.10870394
DO - 10.1080/00021369.1990.10870394
M3 - Article
AN - SCOPUS:0001448728
VL - 54
SP - 2919
EP - 2924
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 11
ER -