D-fructose dehydrogenase-modified carbon paste electrode containing p-benzoquinone as a mediated amperometric fructose sensor

Tokuji Ikeda, Fumio Matsushita, Mitsugi Senda

研究成果: Article

33 引用 (Scopus)

抄録

D-Fructose dehydrogenase was immobilized behind a dialysis membrane on a carbon paste electrode containing p-benzoquinone. The electrode showed a current response to D-fructose due to the p-benzoquinone-mediated bioelectrocatalytic oxidation of D-fructose. The dependence of the current response on the potential applied to the electrode, pH of the solution, and temperature was studied. On this basis, measurements of D-fructose were done at 0.5 V vs. Ag/AgCl, pH 4.5 and 25°C using the fructose dehydrogenase-modified carbon paste electrode. D-Fructose in fruits was measured by this electrode method, in which the interference from L-ascorbic acid was taken into account. A method of elimination of the interference, which uses ascorbate oxidase, is also described.

元の言語English
ページ(範囲)2919-2924
ページ数6
ジャーナルAgricultural and Biological Chemistry
54
発行部数11
DOI
出版物ステータスPublished - 11-1990

Fingerprint

benzoquinones
Ointments
Fructose
electrodes
sensors (equipment)
fructose
Oxidoreductases
Electrodes
Carbon
carbon
Sensors
Ascorbate Oxidase
Dialysis membranes
ascorbate oxidase
dialysis
Fruits
Ascorbic Acid
benzoquinone
Dialysis
Fruit

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

これを引用

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