Decreased expression of a member of the Rho GTPase family, Cdc42Hs, in cells from Tangier disease - The small G protein may play a role in cholesterol efflux

Ken Ichi Hirano, Fumihiko Matsuura, Kosuke Tsukamoto, Zhongyan Zhang, Akifumi Matsuyama, Kenji Takaishi, Ryutaro Komuro, Tadashi Suehiro, Shizuya Yamashita, Yoshimi Takai, Yuji Matsuzawa

研究成果: Article査読

46 被引用数 (Scopus)

抄録

Cholesterol efflux (CE) is the initial and important step of reverse cholesterol transport (RCT), a major protective system against atherosclerosis. However, most of the molecular mechanism for CE still remains to be clarified. In the present study, cDNA subtraction revealed that the expression of a member of the Rho GTPase family, Cdc42Hs, was markedly decreased in both passaged fibroblasts and macrophages (Mφ) from patients with Tangier disease (TD), a rare lipoprotein disorder with reduced CE. This small G protein is known to have many cell biological activities such as rearrangement of actin cytoskeleton and vesicular transport, however the association between this molecule and lipid transport has never been reported. We demonstrate that MDCK cells expressing the dominant negative form of Cdc42Hs had reduced CE, inversely ones expressing the dominant active form had increased CE. From these observations, we would like to raise a novel hypothesis that this type of small G protein may play a role in some steps of CE. To our knowledge, the present study is the first demonstration that the expression of this molecule is altered in cells from human disease. Copyright (C) 2000 Federation of European Biochemical Societies.

本文言語English
ページ(範囲)275-279
ページ数5
ジャーナルFEBS Letters
484
3
DOI
出版ステータスPublished - 10-11-2000
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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