Decreased Expression of MicroRNA-143 and-145 in Human Gastric Cancers

Takeshi Takagi, Akio Iio, Yoshihito Nakagawa, Tomoki Naoe, Nobuhiko Tanigawa, Yukihiro Akao

研究成果: Article

247 引用 (Scopus)

抄録

Objective: Downregulation of specific microRNAs (miRNAs) occurs in human tumors, which suggests a function for miRNAs in tumor suppression. We investigated the role of the miRNAs miR-143 and miR-145 in gastric cancers. Methods: The expression levels of miR-143 and miR-145 in the samples from 43 patients with gastric cancer were determined by real-time PCR using TaqMan assay. The growth inhibitory effect was estimated by the transfection of human gastric cancer cells with the miRNA. Results: The expression levels of miR-143 and-145 were decreased in most human gastric cancers examined, as previously reported to occur in colon tumors. The transfection of human gastric MKN-1 cells with miR-145 resulted in a greater growth inhibitory effect than that with miR-143, results which were contrary to those in colon cancers. In MKN-1 cells, an additive effect on growth inhibition was shown by the combined transfection with miR-143 and miR-145; further, higher sensitivity to 5-fluorouracil was also observed following the transfection with miR-143 or miR-145. The possible candidate target messenger RNAs of miR-145 were identified to be insulin receptor substrate-1 and β-actin. Conclusion: Taken together, these findings suggest that miR-143 and miR-145 act as anti-oncomirs common to gastrointestinal tumors.

元の言語English
ページ(範囲)12-21
ページ数10
ジャーナルOncology
77
発行部数1
DOI
出版物ステータスPublished - 01-07-2009
外部発表Yes

Fingerprint

MicroRNAs
Stomach Neoplasms
Transfection
Neoplasms
Growth
Insulin Receptor Substrate Proteins
Fluorouracil
Colonic Neoplasms
Actins
Real-Time Polymerase Chain Reaction
Stomach
Colon
Down-Regulation
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Oncology

これを引用

Takagi, Takeshi ; Iio, Akio ; Nakagawa, Yoshihito ; Naoe, Tomoki ; Tanigawa, Nobuhiko ; Akao, Yukihiro. / Decreased Expression of MicroRNA-143 and-145 in Human Gastric Cancers. :: Oncology. 2009 ; 巻 77, 番号 1. pp. 12-21.
@article{4b86df8b6cd7420f80f144193f4d3845,
title = "Decreased Expression of MicroRNA-143 and-145 in Human Gastric Cancers",
abstract = "Objective: Downregulation of specific microRNAs (miRNAs) occurs in human tumors, which suggests a function for miRNAs in tumor suppression. We investigated the role of the miRNAs miR-143 and miR-145 in gastric cancers. Methods: The expression levels of miR-143 and miR-145 in the samples from 43 patients with gastric cancer were determined by real-time PCR using TaqMan assay. The growth inhibitory effect was estimated by the transfection of human gastric cancer cells with the miRNA. Results: The expression levels of miR-143 and-145 were decreased in most human gastric cancers examined, as previously reported to occur in colon tumors. The transfection of human gastric MKN-1 cells with miR-145 resulted in a greater growth inhibitory effect than that with miR-143, results which were contrary to those in colon cancers. In MKN-1 cells, an additive effect on growth inhibition was shown by the combined transfection with miR-143 and miR-145; further, higher sensitivity to 5-fluorouracil was also observed following the transfection with miR-143 or miR-145. The possible candidate target messenger RNAs of miR-145 were identified to be insulin receptor substrate-1 and β-actin. Conclusion: Taken together, these findings suggest that miR-143 and miR-145 act as anti-oncomirs common to gastrointestinal tumors.",
author = "Takeshi Takagi and Akio Iio and Yoshihito Nakagawa and Tomoki Naoe and Nobuhiko Tanigawa and Yukihiro Akao",
year = "2009",
month = "7",
day = "1",
doi = "10.1159/000218166",
language = "English",
volume = "77",
pages = "12--21",
journal = "Oncology",
issn = "0030-2414",
publisher = "S. Karger AG",
number = "1",

}

Takagi, T, Iio, A, Nakagawa, Y, Naoe, T, Tanigawa, N & Akao, Y 2009, 'Decreased Expression of MicroRNA-143 and-145 in Human Gastric Cancers', Oncology, 巻. 77, 番号 1, pp. 12-21. https://doi.org/10.1159/000218166

Decreased Expression of MicroRNA-143 and-145 in Human Gastric Cancers. / Takagi, Takeshi; Iio, Akio; Nakagawa, Yoshihito; Naoe, Tomoki; Tanigawa, Nobuhiko; Akao, Yukihiro.

:: Oncology, 巻 77, 番号 1, 01.07.2009, p. 12-21.

研究成果: Article

TY - JOUR

T1 - Decreased Expression of MicroRNA-143 and-145 in Human Gastric Cancers

AU - Takagi, Takeshi

AU - Iio, Akio

AU - Nakagawa, Yoshihito

AU - Naoe, Tomoki

AU - Tanigawa, Nobuhiko

AU - Akao, Yukihiro

PY - 2009/7/1

Y1 - 2009/7/1

N2 - Objective: Downregulation of specific microRNAs (miRNAs) occurs in human tumors, which suggests a function for miRNAs in tumor suppression. We investigated the role of the miRNAs miR-143 and miR-145 in gastric cancers. Methods: The expression levels of miR-143 and miR-145 in the samples from 43 patients with gastric cancer were determined by real-time PCR using TaqMan assay. The growth inhibitory effect was estimated by the transfection of human gastric cancer cells with the miRNA. Results: The expression levels of miR-143 and-145 were decreased in most human gastric cancers examined, as previously reported to occur in colon tumors. The transfection of human gastric MKN-1 cells with miR-145 resulted in a greater growth inhibitory effect than that with miR-143, results which were contrary to those in colon cancers. In MKN-1 cells, an additive effect on growth inhibition was shown by the combined transfection with miR-143 and miR-145; further, higher sensitivity to 5-fluorouracil was also observed following the transfection with miR-143 or miR-145. The possible candidate target messenger RNAs of miR-145 were identified to be insulin receptor substrate-1 and β-actin. Conclusion: Taken together, these findings suggest that miR-143 and miR-145 act as anti-oncomirs common to gastrointestinal tumors.

AB - Objective: Downregulation of specific microRNAs (miRNAs) occurs in human tumors, which suggests a function for miRNAs in tumor suppression. We investigated the role of the miRNAs miR-143 and miR-145 in gastric cancers. Methods: The expression levels of miR-143 and miR-145 in the samples from 43 patients with gastric cancer were determined by real-time PCR using TaqMan assay. The growth inhibitory effect was estimated by the transfection of human gastric cancer cells with the miRNA. Results: The expression levels of miR-143 and-145 were decreased in most human gastric cancers examined, as previously reported to occur in colon tumors. The transfection of human gastric MKN-1 cells with miR-145 resulted in a greater growth inhibitory effect than that with miR-143, results which were contrary to those in colon cancers. In MKN-1 cells, an additive effect on growth inhibition was shown by the combined transfection with miR-143 and miR-145; further, higher sensitivity to 5-fluorouracil was also observed following the transfection with miR-143 or miR-145. The possible candidate target messenger RNAs of miR-145 were identified to be insulin receptor substrate-1 and β-actin. Conclusion: Taken together, these findings suggest that miR-143 and miR-145 act as anti-oncomirs common to gastrointestinal tumors.

UR - http://www.scopus.com/inward/record.url?scp=65549098881&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65549098881&partnerID=8YFLogxK

U2 - 10.1159/000218166

DO - 10.1159/000218166

M3 - Article

C2 - 19439999

AN - SCOPUS:65549098881

VL - 77

SP - 12

EP - 21

JO - Oncology

JF - Oncology

SN - 0030-2414

IS - 1

ER -