抄録
Objective: Downregulation of specific microRNAs (miRNAs) occurs in human tumors, which suggests a function for miRNAs in tumor suppression. We investigated the role of the miRNAs miR-143 and miR-145 in gastric cancers. Methods: The expression levels of miR-143 and miR-145 in the samples from 43 patients with gastric cancer were determined by real-time PCR using TaqMan assay. The growth inhibitory effect was estimated by the transfection of human gastric cancer cells with the miRNA. Results: The expression levels of miR-143 and-145 were decreased in most human gastric cancers examined, as previously reported to occur in colon tumors. The transfection of human gastric MKN-1 cells with miR-145 resulted in a greater growth inhibitory effect than that with miR-143, results which were contrary to those in colon cancers. In MKN-1 cells, an additive effect on growth inhibition was shown by the combined transfection with miR-143 and miR-145; further, higher sensitivity to 5-fluorouracil was also observed following the transfection with miR-143 or miR-145. The possible candidate target messenger RNAs of miR-145 were identified to be insulin receptor substrate-1 and β-actin. Conclusion: Taken together, these findings suggest that miR-143 and miR-145 act as anti-oncomirs common to gastrointestinal tumors.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 12-21 |
| ページ数 | 10 |
| ジャーナル | Oncology |
| 巻 | 77 |
| 発行部数 | 1 |
| DOI | |
| 出版物ステータス | Published - 01-07-2009 |
| 外部発表 | Yes |
Fingerprint
All Science Journal Classification (ASJC) codes
- Cancer Research
- Oncology
これを引用
}
Decreased Expression of MicroRNA-143 and-145 in Human Gastric Cancers. / Takagi, Takeshi; Iio, Akio; Nakagawa, Yoshihito; Naoe, Tomoki; Tanigawa, Nobuhiko; Akao, Yukihiro.
：: Oncology, 巻 77, 番号 1, 01.07.2009, p. 12-21.研究成果: Article
TY - JOUR
T1 - Decreased Expression of MicroRNA-143 and-145 in Human Gastric Cancers
AU - Takagi, Takeshi
AU - Iio, Akio
AU - Nakagawa, Yoshihito
AU - Naoe, Tomoki
AU - Tanigawa, Nobuhiko
AU - Akao, Yukihiro
PY - 2009/7/1
Y1 - 2009/7/1
N2 - Objective: Downregulation of specific microRNAs (miRNAs) occurs in human tumors, which suggests a function for miRNAs in tumor suppression. We investigated the role of the miRNAs miR-143 and miR-145 in gastric cancers. Methods: The expression levels of miR-143 and miR-145 in the samples from 43 patients with gastric cancer were determined by real-time PCR using TaqMan assay. The growth inhibitory effect was estimated by the transfection of human gastric cancer cells with the miRNA. Results: The expression levels of miR-143 and-145 were decreased in most human gastric cancers examined, as previously reported to occur in colon tumors. The transfection of human gastric MKN-1 cells with miR-145 resulted in a greater growth inhibitory effect than that with miR-143, results which were contrary to those in colon cancers. In MKN-1 cells, an additive effect on growth inhibition was shown by the combined transfection with miR-143 and miR-145; further, higher sensitivity to 5-fluorouracil was also observed following the transfection with miR-143 or miR-145. The possible candidate target messenger RNAs of miR-145 were identified to be insulin receptor substrate-1 and β-actin. Conclusion: Taken together, these findings suggest that miR-143 and miR-145 act as anti-oncomirs common to gastrointestinal tumors.
AB - Objective: Downregulation of specific microRNAs (miRNAs) occurs in human tumors, which suggests a function for miRNAs in tumor suppression. We investigated the role of the miRNAs miR-143 and miR-145 in gastric cancers. Methods: The expression levels of miR-143 and miR-145 in the samples from 43 patients with gastric cancer were determined by real-time PCR using TaqMan assay. The growth inhibitory effect was estimated by the transfection of human gastric cancer cells with the miRNA. Results: The expression levels of miR-143 and-145 were decreased in most human gastric cancers examined, as previously reported to occur in colon tumors. The transfection of human gastric MKN-1 cells with miR-145 resulted in a greater growth inhibitory effect than that with miR-143, results which were contrary to those in colon cancers. In MKN-1 cells, an additive effect on growth inhibition was shown by the combined transfection with miR-143 and miR-145; further, higher sensitivity to 5-fluorouracil was also observed following the transfection with miR-143 or miR-145. The possible candidate target messenger RNAs of miR-145 were identified to be insulin receptor substrate-1 and β-actin. Conclusion: Taken together, these findings suggest that miR-143 and miR-145 act as anti-oncomirs common to gastrointestinal tumors.
UR - http://www.scopus.com/inward/record.url?scp=65549098881&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65549098881&partnerID=8YFLogxK
U2 - 10.1159/000218166
DO - 10.1159/000218166
M3 - Article
C2 - 19439999
AN - SCOPUS:65549098881
VL - 77
SP - 12
EP - 21
JO - Oncology
JF - Oncology
SN - 0030-2414
IS - 1
ER -