TY - JOUR
T1 - Decreased l-tryptophan concentration in distinctive brain regions of mice treated repeatedly with phencyclidine Amino Acid Analysis
AU - Iizuka, Hideaki
AU - Watanabe, Shoko
AU - Koshikawa, Mayuko
AU - Matsumoto, Yurie
AU - Aoyama, Yuki
AU - Ichiba, Hideaki
AU - Nabeshima, Toshitaka
AU - Fukushima, Takeshi
N1 - Funding Information:
Acknowledgments The present study was financially supported by grant-in-aid for Scientific Researches (C) (23617027) and Scientific Researches (C) (22590147) from the Japan Society for the Promotion of Science, the Ministry of Education, Culture, Sports, Science, and Technology of Japan. The authors thank Prof. Furukawa, Meijo University, for the synthesis of PCP and Drs. H. Yamada and S. Iwasa, Toho University, for their helpful discussions on this study.
PY - 2013/10
Y1 - 2013/10
N2 - It has been reported that repeated phencyclidine (PCP) treatment induces schizophrenia-like behavior in mice. l-Tryptophan (Trp) concentrations in brain tissues of control (n = 8) and PCP-treated mice (10 mg/kg/day, s.c., 14 days, n = 10) were determined using high-performance liquid chromatography (HPLC) with fluorescence detection. The HPLC method involved pre-column fluorescence derivatization with (R)-(-)-4-(N,N-dimethylaminosulfonyl)-7-(3- isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole (DBD-PyNCS). Eight different parts of the brain, namely, the frontal cortex, nucleus accumbens, striatum, hippocampus, amygdala, thalamus, hypothalamus, and cerebellum, of both groups were investigated. A significant decrease in the l-Trp concentration in the nucleus accumbens (p = 0.024) and hippocampus (p = 0.027) was observed in PCP-treated mice, suggesting that alteration of the l-Trp metabolism might occur in these brain parts.
AB - It has been reported that repeated phencyclidine (PCP) treatment induces schizophrenia-like behavior in mice. l-Tryptophan (Trp) concentrations in brain tissues of control (n = 8) and PCP-treated mice (10 mg/kg/day, s.c., 14 days, n = 10) were determined using high-performance liquid chromatography (HPLC) with fluorescence detection. The HPLC method involved pre-column fluorescence derivatization with (R)-(-)-4-(N,N-dimethylaminosulfonyl)-7-(3- isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole (DBD-PyNCS). Eight different parts of the brain, namely, the frontal cortex, nucleus accumbens, striatum, hippocampus, amygdala, thalamus, hypothalamus, and cerebellum, of both groups were investigated. A significant decrease in the l-Trp concentration in the nucleus accumbens (p = 0.024) and hippocampus (p = 0.027) was observed in PCP-treated mice, suggesting that alteration of the l-Trp metabolism might occur in these brain parts.
UR - https://www.scopus.com/pages/publications/84885204289
UR - https://www.scopus.com/pages/publications/84885204289#tab=citedBy
U2 - 10.1007/s00216-013-7010-2
DO - 10.1007/s00216-013-7010-2
M3 - Article
C2 - 23665637
AN - SCOPUS:84885204289
SN - 1618-2642
VL - 405
SP - 8137
EP - 8141
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 25
ER -