Degradation of phosphorylated p53 by viral protein-ECS E3 ligase complex

Yoshitaka Sato, Takumi Kamura, Noriko Shirata, Takayuki Murata, Ayumi Kudoh, Satoko Iwahori, Sanae Nakayama, Hiroki Isomura, Yukihiro Nishiyama, Tatsuya Tsurumi

研究成果: Article査読

88 被引用数 (Scopus)

抄録

p53-signaling is modulated by viruses to establish a host cellular environment advantageous for their propagation. The Epstein-Barr virus (EBV) lytic program induces phosphorylation of p53, which prevents interaction with MDM2. Here, we show that induction of EBV lytic program leads to degradation of p53 via an ubiquitin-proteasome pathway independent of MDM2. The BZLF1 protein directly functions as an adaptor component of the ECS (Elongin B/C-Cul2/5-SOCS-box protein) ubiquitin ligase complex targeting p53 for degradation. Intringuingly, C-terminal phosphorylation of p53 resulting from activated DNA damage response by viral lytic replication enhances its binding to BZLF1 protein. Purified BZLF1 proteinassociated ECS could be shown to catalyze ubiquitination of phospho-mimetic p53 more efficiently than the wild-type in vitro. The compensation of p53 at middle and late stages of the lytic infection inhibits viral DNA replication and production during lytic infection, suggesting that the degradation of p53 is required for efficient viral propagation. Taken together, these findings demonstrate a role for the BZLF1 protein-associated ECS ligase complex in regulation of p53 phosphorylated by activated DNA damage signaling during viral lytic infection.

本文言語English
論文番号e1000530
ジャーナルPLoS Pathogens
5
7
DOI
出版ステータスPublished - 07-2009
外部発表はい

All Science Journal Classification (ASJC) codes

  • 寄生虫科
  • 微生物学
  • 免疫学
  • 分子生物学
  • 遺伝学
  • ウイルス学

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