Delayed neuronal cell death in brainstem after transient brainstem ischemia in gerbils

Fang Cao, Ryuji Hata, Pengxiang Zhu, Shoichiro Takeda, Tadashi Yoshida, Nobuhiro Hakuba, Masahiro Sakanaka, Kiyofumi Gyo

研究成果: Article

2 引用 (Scopus)

抄録

Background: Because of the lack of reproducible brainstem ischemia models in rodents, the temporal profile of ischemic lesions in the brainstem after transient brainstem ischemia has not been evaluated intensively. Previously, we produced a reproducible brainstem ischemia model of Mongolian gerbils. Here, we showed the temporal profile of ischemic lesions after transient brainstem ischemia.Results: Brainstem ischemia was produced by occlusion of the bilateral vertebral arteries just before their entry into the transverse foramina of the cervical vertebrae of Mongolian gerbils. Animals were subjected to brainstem ischemia for 15 min, and then reperfused for 0 d (just after ischemia), 1 d, 3 d and 7 d (n = 4 in each group). Sham-operated animals (n = 4) were used as control. After deep anesthesia, the gerbils were perfused with fixative for immunohistochemical investigation. Ischemic lesions were detected by immunostaining for microtubule-associated protein 2 (MAP2). Just after 15-min brainstem ischemia, ischemic lesions were detected in the lateral vestibular nucleus and the ventral part of the spinal trigeminal nucleus, and these ischemic lesions disappeared one day after reperfusion in all animals examined. However, 3 days and 7 days after reperfusion, ischemic lesions appeared again and clusters of ionized calcium-binding adapter molecule-1(IBA-1)-positive cells were detected in the same areas in all animals.Conclusion: These results suggest that delayed neuronal cell death took place in the brainstem after transient brainstem ischemia in gerbils.

元の言語English
記事番号115
ジャーナルBMC Neuroscience
11
DOI
出版物ステータスPublished - 14-09-2010

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Gerbillinae
Transient Ischemic Attack
Brain Stem
Cell Death
Ischemia
Reperfusion
Lateral Vestibular Nucleus
Spinal Trigeminal Nucleus
Cervical Vertebrae
Fixatives
Microtubule-Associated Proteins
Vertebral Artery
Rodentia
Anesthesia
Calcium

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Cellular and Molecular Neuroscience

これを引用

Cao, Fang ; Hata, Ryuji ; Zhu, Pengxiang ; Takeda, Shoichiro ; Yoshida, Tadashi ; Hakuba, Nobuhiro ; Sakanaka, Masahiro ; Gyo, Kiyofumi. / Delayed neuronal cell death in brainstem after transient brainstem ischemia in gerbils. :: BMC Neuroscience. 2010 ; 巻 11.
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abstract = "Background: Because of the lack of reproducible brainstem ischemia models in rodents, the temporal profile of ischemic lesions in the brainstem after transient brainstem ischemia has not been evaluated intensively. Previously, we produced a reproducible brainstem ischemia model of Mongolian gerbils. Here, we showed the temporal profile of ischemic lesions after transient brainstem ischemia.Results: Brainstem ischemia was produced by occlusion of the bilateral vertebral arteries just before their entry into the transverse foramina of the cervical vertebrae of Mongolian gerbils. Animals were subjected to brainstem ischemia for 15 min, and then reperfused for 0 d (just after ischemia), 1 d, 3 d and 7 d (n = 4 in each group). Sham-operated animals (n = 4) were used as control. After deep anesthesia, the gerbils were perfused with fixative for immunohistochemical investigation. Ischemic lesions were detected by immunostaining for microtubule-associated protein 2 (MAP2). Just after 15-min brainstem ischemia, ischemic lesions were detected in the lateral vestibular nucleus and the ventral part of the spinal trigeminal nucleus, and these ischemic lesions disappeared one day after reperfusion in all animals examined. However, 3 days and 7 days after reperfusion, ischemic lesions appeared again and clusters of ionized calcium-binding adapter molecule-1(IBA-1)-positive cells were detected in the same areas in all animals.Conclusion: These results suggest that delayed neuronal cell death took place in the brainstem after transient brainstem ischemia in gerbils.",
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Delayed neuronal cell death in brainstem after transient brainstem ischemia in gerbils. / Cao, Fang; Hata, Ryuji; Zhu, Pengxiang; Takeda, Shoichiro; Yoshida, Tadashi; Hakuba, Nobuhiro; Sakanaka, Masahiro; Gyo, Kiyofumi.

:: BMC Neuroscience, 巻 11, 115, 14.09.2010.

研究成果: Article

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AU - Hata, Ryuji

AU - Zhu, Pengxiang

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AU - Yoshida, Tadashi

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AU - Sakanaka, Masahiro

AU - Gyo, Kiyofumi

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