Demonstration of lipid A-binding proteins on murine lymphoma cells using R-mutant Gram-negative bacteria as a detector

I. Nakashima, T. Kubota, Y. Zhang, T. Yoshida, R. Inagi, K. Isobe, F. Nagase, M. Ohta, N. Kato, T. Yokochi

研究成果: Article査読

抄録

Some R-mutant Escherichia coli and Salmonella heavily adhered to murine lymphoma cells of B cell and T cell lineages. This adhesion was primarily mediated by membrane-localized proteins on tumor cells, which bind the polymyxin B-reactive hydrophilic structure of lipid A on bacteria. SDS-PAGE analysis of tumor cell membranes showed that proteins or glycoproteins of MW = around 45Kd, 25-35Kd and around 15Kd preferentially bind lipid A. Various lymphoma cell lines binding the bacteria at different levels possessed lipid A-binding proteins of slightly different compositions. We conclude that lymphoma cells carry not a single but a group of lipid A-binding proteins in their membranes.

本文言語English
ページ(範囲)87-94
ページ数8
ジャーナルAPMIS
98
1
DOI
出版ステータスPublished - 1990
外部発表はい

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Immunology and Allergy
  • Microbiology (medical)

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