Designing multiplex PCR system of Campylobacter jejuni for efficient typing by improving monoplex PCR binary typing method

Kazuhiro Yamada, Ami Ibata, Masahiro Suzuki, Masakado Matsumoto, Teruo Yamashita, Hiroko Minagawa, Ryuichiro Kurane

研究成果: Article査読

5 被引用数 (Scopus)

抄録

Campylobacter jejuni is responsible for the majority of Campylobacter infections. As the molecular epidemiological study of outbreaks, pulsed-field gel electrophoresis (PFGE) is performed in general. But PFGE has several problems. PCR binary typing (P-BIT) method is a typing method for Campylobacter spp. that was recently developed, and was reported to have a similar discriminatory power and stability to those of PFGE. We modified the P-BIT method from 18 monoplex PCRs to two multiplex PCR systems (mP-BIT). The same results were obtained from monoplex PCRs using original primers and multiplex PCR in the representative isolates. The mP-BIT can analyze 48 strains at a time by using 96-well PCR systems and can identify C. jejuni because mP-BIT includes C. jejuni marker. The typing of the isolates by the mP-BIT and PFGE demonstrated generally concordant results and the mP-BIT method (D = 0.980) has a similar discriminatory power to that of PFGE with SmaI digest (D = 0.975) or KpnI digest (D = 0.987) as with original article. The mP-BIT method is quick, simple and easy, and comes to be able to perform it at low cost by having become a multiplex PCR system. Therefore, the mP-BIT method with two multiplex PCR systems has high potential for a rapid first-line surveillance typing assay of C. jejuni and can be used for routine surveillance and outbreak investigations of C. jejuni in the future.

本文言語English
ページ(範囲)50-54
ページ数5
ジャーナルJournal of Infection and Chemotherapy
21
1
DOI
出版ステータスPublished - 05-06-2014
外部発表はい

All Science Journal Classification (ASJC) codes

  • 微生物学(医療)
  • 感染症
  • 薬理学(医学)

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