Detection and identification of oral anaerobes in intraoperative bronchial fluids of patients with pulmonary carcinoma

Ayako Hasegawa, Takuichi Sato, Yasushi Hoshikawa, Naoko Ishida, Naoko Tanda, Yoshiaki Kawamura, Takashi Kondo, Nobuhiro Takahashi

研究成果: Article

7 引用 (Scopus)

抄録

Postoperative pneumonia may occur when upper respiratory tract protective reflexes such as cough and/or swallowing reflexes are impaired; thus, silent aspiration of oral bacteria may be a causative factor in postoperative pneumonia. This study aimed to quantify and identify bacteria in intraoperative bronchial fluids and to evaluate the relationship between impairment of cough/swallowing reflexes and silent aspiration of oral bacteria in elderly patients. After obtaining informed consent, cough and swallowing reflexes were assessed using an ultrasonic nebulizer and a nasal catheter, respectively. Using a micro-sampling probe, intraoperative bronchial fluids were collected from nine subjects with pulmonary carcinoma and cultured anaerobically on blood agar plates. After 7 days, CFUs were counted and isolated bacteria were identified by 16S rRNA gene sequencing. Four subjects (aged 71.0±8.4 years) had impaired swallowing reflexes with normal cough reflexes, whereas five subjects (73.6±6.5 years) had normal cough and swallowing reflexes. The bacterial counts (mean CFU±SD) tended to be higher in intraoperative bronchial fluids of subjects with impaired swallowing reflexes ([5.1±7.7]×105) than in those of subjects with normal reflexes ([1.2±1.9]×105); however, this difference was not statistically significant. Predominant isolates from intraoperative bronchial fluids were Streptococcus (41.8%), Veillonella (11.4%), Gemella (8.9%), Porphyromonas (7.6%), Olsenella (6.3%) and Eikenella (6.3%). These findings indicate that intraoperative bronchial fluids contain bacteria, probably derived from the oral microbiota, and suggest that silent aspiration of oral bacteria occurs in elderly patients irrespective of impairment of swallowing reflex.

元の言語English
ページ(範囲)375-381
ページ数7
ジャーナルMicrobiology and Immunology
58
発行部数7
DOI
出版物ステータスPublished - 01-01-2014

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Reflex
Deglutition
Carcinoma
Lung
Cough
Bacteria
Eikenella
Pneumonia
Gemella
Porphyromonas
Veillonella
Bacterial Load
Nebulizers and Vaporizers
Microbiota
Streptococcus
Informed Consent
rRNA Genes
Nose
Ultrasonics
Respiratory System

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

これを引用

Hasegawa, Ayako ; Sato, Takuichi ; Hoshikawa, Yasushi ; Ishida, Naoko ; Tanda, Naoko ; Kawamura, Yoshiaki ; Kondo, Takashi ; Takahashi, Nobuhiro. / Detection and identification of oral anaerobes in intraoperative bronchial fluids of patients with pulmonary carcinoma. :: Microbiology and Immunology. 2014 ; 巻 58, 番号 7. pp. 375-381.
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abstract = "Postoperative pneumonia may occur when upper respiratory tract protective reflexes such as cough and/or swallowing reflexes are impaired; thus, silent aspiration of oral bacteria may be a causative factor in postoperative pneumonia. This study aimed to quantify and identify bacteria in intraoperative bronchial fluids and to evaluate the relationship between impairment of cough/swallowing reflexes and silent aspiration of oral bacteria in elderly patients. After obtaining informed consent, cough and swallowing reflexes were assessed using an ultrasonic nebulizer and a nasal catheter, respectively. Using a micro-sampling probe, intraoperative bronchial fluids were collected from nine subjects with pulmonary carcinoma and cultured anaerobically on blood agar plates. After 7 days, CFUs were counted and isolated bacteria were identified by 16S rRNA gene sequencing. Four subjects (aged 71.0±8.4 years) had impaired swallowing reflexes with normal cough reflexes, whereas five subjects (73.6±6.5 years) had normal cough and swallowing reflexes. The bacterial counts (mean CFU±SD) tended to be higher in intraoperative bronchial fluids of subjects with impaired swallowing reflexes ([5.1±7.7]×105) than in those of subjects with normal reflexes ([1.2±1.9]×105); however, this difference was not statistically significant. Predominant isolates from intraoperative bronchial fluids were Streptococcus (41.8{\%}), Veillonella (11.4{\%}), Gemella (8.9{\%}), Porphyromonas (7.6{\%}), Olsenella (6.3{\%}) and Eikenella (6.3{\%}). These findings indicate that intraoperative bronchial fluids contain bacteria, probably derived from the oral microbiota, and suggest that silent aspiration of oral bacteria occurs in elderly patients irrespective of impairment of swallowing reflex.",
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Detection and identification of oral anaerobes in intraoperative bronchial fluids of patients with pulmonary carcinoma. / Hasegawa, Ayako; Sato, Takuichi; Hoshikawa, Yasushi; Ishida, Naoko; Tanda, Naoko; Kawamura, Yoshiaki; Kondo, Takashi; Takahashi, Nobuhiro.

:: Microbiology and Immunology, 巻 58, 番号 7, 01.01.2014, p. 375-381.

研究成果: Article

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AU - Hasegawa, Ayako

AU - Sato, Takuichi

AU - Hoshikawa, Yasushi

AU - Ishida, Naoko

AU - Tanda, Naoko

AU - Kawamura, Yoshiaki

AU - Kondo, Takashi

AU - Takahashi, Nobuhiro

PY - 2014/1/1

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N2 - Postoperative pneumonia may occur when upper respiratory tract protective reflexes such as cough and/or swallowing reflexes are impaired; thus, silent aspiration of oral bacteria may be a causative factor in postoperative pneumonia. This study aimed to quantify and identify bacteria in intraoperative bronchial fluids and to evaluate the relationship between impairment of cough/swallowing reflexes and silent aspiration of oral bacteria in elderly patients. After obtaining informed consent, cough and swallowing reflexes were assessed using an ultrasonic nebulizer and a nasal catheter, respectively. Using a micro-sampling probe, intraoperative bronchial fluids were collected from nine subjects with pulmonary carcinoma and cultured anaerobically on blood agar plates. After 7 days, CFUs were counted and isolated bacteria were identified by 16S rRNA gene sequencing. Four subjects (aged 71.0±8.4 years) had impaired swallowing reflexes with normal cough reflexes, whereas five subjects (73.6±6.5 years) had normal cough and swallowing reflexes. The bacterial counts (mean CFU±SD) tended to be higher in intraoperative bronchial fluids of subjects with impaired swallowing reflexes ([5.1±7.7]×105) than in those of subjects with normal reflexes ([1.2±1.9]×105); however, this difference was not statistically significant. Predominant isolates from intraoperative bronchial fluids were Streptococcus (41.8%), Veillonella (11.4%), Gemella (8.9%), Porphyromonas (7.6%), Olsenella (6.3%) and Eikenella (6.3%). These findings indicate that intraoperative bronchial fluids contain bacteria, probably derived from the oral microbiota, and suggest that silent aspiration of oral bacteria occurs in elderly patients irrespective of impairment of swallowing reflex.

AB - Postoperative pneumonia may occur when upper respiratory tract protective reflexes such as cough and/or swallowing reflexes are impaired; thus, silent aspiration of oral bacteria may be a causative factor in postoperative pneumonia. This study aimed to quantify and identify bacteria in intraoperative bronchial fluids and to evaluate the relationship between impairment of cough/swallowing reflexes and silent aspiration of oral bacteria in elderly patients. After obtaining informed consent, cough and swallowing reflexes were assessed using an ultrasonic nebulizer and a nasal catheter, respectively. Using a micro-sampling probe, intraoperative bronchial fluids were collected from nine subjects with pulmonary carcinoma and cultured anaerobically on blood agar plates. After 7 days, CFUs were counted and isolated bacteria were identified by 16S rRNA gene sequencing. Four subjects (aged 71.0±8.4 years) had impaired swallowing reflexes with normal cough reflexes, whereas five subjects (73.6±6.5 years) had normal cough and swallowing reflexes. The bacterial counts (mean CFU±SD) tended to be higher in intraoperative bronchial fluids of subjects with impaired swallowing reflexes ([5.1±7.7]×105) than in those of subjects with normal reflexes ([1.2±1.9]×105); however, this difference was not statistically significant. Predominant isolates from intraoperative bronchial fluids were Streptococcus (41.8%), Veillonella (11.4%), Gemella (8.9%), Porphyromonas (7.6%), Olsenella (6.3%) and Eikenella (6.3%). These findings indicate that intraoperative bronchial fluids contain bacteria, probably derived from the oral microbiota, and suggest that silent aspiration of oral bacteria occurs in elderly patients irrespective of impairment of swallowing reflex.

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