TY - JOUR
T1 - Detection of herpesvirus DNAs in perilymph obtained from patients with sensorineural hearing loss by real-time polymerase chain reaction
AU - Sugiura, Saiko
AU - Yoshikawa, Tetsushi
AU - Nishiyama, Yukihiro
AU - Morishita, Yoshihiro
AU - Sato, Eisuke
AU - Beppu, Reiko
AU - Hattori, Taku
AU - Nakashima, Tsutomu
N1 - Funding Information:
We thank D. Bundy for helping to produce the CAM4 simulations, A. Subramanian for stimulating discussions, and three anonymous reviewers for their constructive feedback. P.W. was supported by European Research Council grant 291406. The EC-Earth simulations were performed in the framework of the PRACE project Climate-SPHINX: S.C., P.D., and J.v.H. acknowledge PRACE for awarding access to the SuperMUC resource based in Germany at LRZ. S.C. and J.v.H. also acknowledge funding received from the PRIMAVERA project (European Commission under grant agreement 641727 of the Horizon 2020 research program). J.v.H. also acknowledges funding from the European Union's Horizon 2020 research and innovation program under grant agreement 641816 (CRESCENDO) and by the Italian project of Interest ?NextData? of the Italian Ministry for Education, University and Research. Additionally, P.D. was supported by funding from the European Union's Horizon 2020 research and innovation program COGNAC under the European Union Marie Sk?odowska-Curie grant agreement 654942. C.S. was supported by the Joint UK BEIS/Defra Met Office Hadley Centre Climate Programme (GA01101). A.W. was supported by the EU FP7 funded project SPECS, grant agreement 308378. The code and data used to perform the data analysis are available from Peter Watson ([email protected]) upon request.
PY - 2004/12
Y1 - 2004/12
N2 - Objectives/Hypothesis: Perilymph and peripheral blood mononuclear cells (PBMCs) from patients with bilateral severe sensorineural hearing loss (SNHL) were evaluated for the presence of DNA from cytomegalovirus (CMV), herpes simplex virus (HSV), and human herpesvirus (HHV)6. Study Design: A prospective clinical study. Methods: The subjects were 14 patients who underwent cochlear implantation and 1 patient who underwent gentamicin injection in the inner ear. We attempted to detect viral DNA from perilymph and PBMCs by real-time polymerase chain reaction (rtPCR). Results: CMV DNA was detected in two perilymph specimens obtained from patients who were diagnosed as congenitally symptomatic CMV infection, although no CMV DNA was detected in PB-MCs. Neither HSV DNA nor HHV6 DNA was detected in any other perilymph specimens. CMV DNA was detected in three PBMC samples, HSV DNA was detected in two samples, and HHV6 DNA was detected in six samples. Conclusion: CMV may persistently infect the inner ear of patients with congenital CMV infection, and rtPCR analysis may prove to be a valuable tool for investigating the etiology of SNHL.
AB - Objectives/Hypothesis: Perilymph and peripheral blood mononuclear cells (PBMCs) from patients with bilateral severe sensorineural hearing loss (SNHL) were evaluated for the presence of DNA from cytomegalovirus (CMV), herpes simplex virus (HSV), and human herpesvirus (HHV)6. Study Design: A prospective clinical study. Methods: The subjects were 14 patients who underwent cochlear implantation and 1 patient who underwent gentamicin injection in the inner ear. We attempted to detect viral DNA from perilymph and PBMCs by real-time polymerase chain reaction (rtPCR). Results: CMV DNA was detected in two perilymph specimens obtained from patients who were diagnosed as congenitally symptomatic CMV infection, although no CMV DNA was detected in PB-MCs. Neither HSV DNA nor HHV6 DNA was detected in any other perilymph specimens. CMV DNA was detected in three PBMC samples, HSV DNA was detected in two samples, and HHV6 DNA was detected in six samples. Conclusion: CMV may persistently infect the inner ear of patients with congenital CMV infection, and rtPCR analysis may prove to be a valuable tool for investigating the etiology of SNHL.
UR - http://www.scopus.com/inward/record.url?scp=10044246339&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=10044246339&partnerID=8YFLogxK
U2 - 10.1097/01.mlg.0000149466.80703.55
DO - 10.1097/01.mlg.0000149466.80703.55
M3 - Article
C2 - 15564852
AN - SCOPUS:10044246339
SN - 0023-852X
VL - 114
SP - 2235
EP - 2238
JO - Laryngoscope
JF - Laryngoscope
IS - 12
ER -