Detection of high-risk carbapenem-resistant Klebsiella pneumoniae and Enterobacter cloacae isolates using volatile molecular profiles

Christiaan A. Rees, Mavra Nasir, Agnieszka Smolinska, Alexa E. Lewis, Katherine R. Kane, Shannon E. Kossmann, Orkan Sezer, Paola C. Zucchi, Yohei Doi, Elizabeth B. Hirsch, Jane E. Hill

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Infections caused by carbapenem-resistant Enterobacteriaceae (CRE) are alarming in the clinical setting, as CRE isolates often exhibit resistance to most clinically-available antibiotics. Klebsiella pneumoniae carbapenemase (KPC) is the most common carbapenemase carried by CRE in North America and Europe, frequently detected in isolates of K. pneumoniae, Escherichia coli, and Enterobacter cloacae. Notably, KPC-expressing strains often arise from clonal lineages, with sequence type 258 (ST258) representing the dominant lineage in K. pneumoniae, ST131 in E. coli, and ST78 and ST171 in E. cloacae. Prior studies have demonstrated that carbapenem-resistant K. pneumoniae differs from carbapenem-susceptible K. pneumoniae at both the transcriptomic and soluble metabolomic levels. In the present study, we sought to determine whether carbapenem-resistant and carbapenem-susceptible isolates of K. pneumoniae, E. coli, and E. cloacae produce distinct volatile metabolic profiles. We were able to identify a volatile metabolic fingerprint that could discriminate between CRE and non-CRE with an area under the receiver operating characteristic curve (AUROC) as high as 0.912. Species-specific AUROCs were as high as 0.988 for K. pneumoniae and 1.000 for E. cloacae. Paradoxically, curing of KPC-expressing plasmids from a subset of K. pneumoniae isolates further accentuated the metabolic differences observed between ST258 and non-ST258.

元の言語English
記事番号13297
ジャーナルScientific reports
8
発行部数1
DOI
出版物ステータスPublished - 01-12-2018
外部発表Yes

All Science Journal Classification (ASJC) codes

  • General

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    Rees, C. A., Nasir, M., Smolinska, A., Lewis, A. E., Kane, K. R., Kossmann, S. E., Sezer, O., Zucchi, P. C., Doi, Y., Hirsch, E. B., & Hill, J. E. (2018). Detection of high-risk carbapenem-resistant Klebsiella pneumoniae and Enterobacter cloacae isolates using volatile molecular profiles. Scientific reports, 8(1), [13297]. https://doi.org/10.1038/s41598-018-31543-x