抄録
Optical near-field enhancement at a surface where a total internal reflection occurs is an effective phenomenon that can enhance optical signals from markers conjugated with biological materials on the surface. The enhanced signal can be an effective tool to detect trace amounts of a biological substance. An external force-assisted near-field illumination biosensor is based on a sandwich assay that uses magnetic particles instead of a sensor surface to capture the targets. This sensing method detects light signals from targets sandwiched between magnetic particles and optical markers. The signal of the sandwiched target can be easily distinguished from optical noise by observing the movement of the signal induced by an external magnetic field. In this study, we improved the detection ability by using 60 nm ∅ gold nanoparticles as markers. Norovirus virus-like particles were added to a buffer or a contaminated water sample and successfully detected using this system.
| 元の言語 | English |
|---|---|
| 記事番号 | 071005 |
| ジャーナル | Japanese Journal of Applied Physics |
| 巻 | 58 |
| 発行部数 | 7 |
| DOI | |
| 出版物ステータス | Published - 01-01-2019 |
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All Science Journal Classification (ASJC) codes
- Engineering(all)
- Physics and Astronomy(all)
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Detection of norovirus-like particles with an external force-assisted near-field illumination biosensor. / Yasuura, Masato; Shirato, Haruko; Higo-Moriguchi, Kyoko; Fujimaki, Makoto.
：: Japanese Journal of Applied Physics, 巻 58, 番号 7, 071005, 01.01.2019.研究成果: Article
TY - JOUR
T1 - Detection of norovirus-like particles with an external force-assisted near-field illumination biosensor
AU - Yasuura, Masato
AU - Shirato, Haruko
AU - Higo-Moriguchi, Kyoko
AU - Fujimaki, Makoto
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Optical near-field enhancement at a surface where a total internal reflection occurs is an effective phenomenon that can enhance optical signals from markers conjugated with biological materials on the surface. The enhanced signal can be an effective tool to detect trace amounts of a biological substance. An external force-assisted near-field illumination biosensor is based on a sandwich assay that uses magnetic particles instead of a sensor surface to capture the targets. This sensing method detects light signals from targets sandwiched between magnetic particles and optical markers. The signal of the sandwiched target can be easily distinguished from optical noise by observing the movement of the signal induced by an external magnetic field. In this study, we improved the detection ability by using 60 nm ∅ gold nanoparticles as markers. Norovirus virus-like particles were added to a buffer or a contaminated water sample and successfully detected using this system.
AB - Optical near-field enhancement at a surface where a total internal reflection occurs is an effective phenomenon that can enhance optical signals from markers conjugated with biological materials on the surface. The enhanced signal can be an effective tool to detect trace amounts of a biological substance. An external force-assisted near-field illumination biosensor is based on a sandwich assay that uses magnetic particles instead of a sensor surface to capture the targets. This sensing method detects light signals from targets sandwiched between magnetic particles and optical markers. The signal of the sandwiched target can be easily distinguished from optical noise by observing the movement of the signal induced by an external magnetic field. In this study, we improved the detection ability by using 60 nm ∅ gold nanoparticles as markers. Norovirus virus-like particles were added to a buffer or a contaminated water sample and successfully detected using this system.
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UR - http://www.scopus.com/inward/citedby.url?scp=85070781452&partnerID=8YFLogxK
U2 - 10.7567/1347-4065/ab29e2
DO - 10.7567/1347-4065/ab29e2
M3 - Article
AN - SCOPUS:85070781452
VL - 58
JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
SN - 0021-4922
IS - 7
M1 - 071005
ER -