TY - JOUR
T1 - Detection of norovirus virus-like particles using a surface plasmon resonance-assisted fluoroimmunosensor optimized for quantum dot fluorescent labels
AU - Ashiba, Hiroki
AU - Sugiyama, Yuki
AU - Wang, Xiaomin
AU - Shirato, Haruko
AU - Higo-Moriguchi, Kyoko
AU - Taniguchi, Koki
AU - Ohki, Yoshimichi
AU - Fujimaki, Makoto
N1 - Publisher Copyright:
© 2016 The Authors
PY - 2017/7/15
Y1 - 2017/7/15
N2 - A highly sensitive biosensor to detect norovirus in environment is desired to prevent the spread of infection. In this study, we investigated a design of surface plasmon resonance (SPR)-assisted fluoroimmunosensor to increase its sensitivity and performed detection of norovirus virus-like particles (VLPs). A quantum dot fluorescent dye was employed because of its large Stokes shift. The sensor design was optimized for the CdSe-ZnS-based quantum dots. The optimal design was applied to a simple SPR-assisted fluoroimmunosensor that uses a sensor chip equipped with a V-shaped trench. Excitation efficiency of the quantum dots, degree of electric field enhancement by SPR, and intensity of autofluorescence of a substrate of the sensor chip were theoretically and experimentally evaluated to maximize the signal-to-noise ratio. As the result, an excitation wavelength of 390 nm was selected to excite SPR on an Al film of the sensor chip. The sandwich assay of norovirus VLPs was performed using the designed sensor. Minimum detectable concentration of 0.01 ng/mL, which corresponds to 100 virus-like particles included in the detection region of the V-trench, was demonstrated.
AB - A highly sensitive biosensor to detect norovirus in environment is desired to prevent the spread of infection. In this study, we investigated a design of surface plasmon resonance (SPR)-assisted fluoroimmunosensor to increase its sensitivity and performed detection of norovirus virus-like particles (VLPs). A quantum dot fluorescent dye was employed because of its large Stokes shift. The sensor design was optimized for the CdSe-ZnS-based quantum dots. The optimal design was applied to a simple SPR-assisted fluoroimmunosensor that uses a sensor chip equipped with a V-shaped trench. Excitation efficiency of the quantum dots, degree of electric field enhancement by SPR, and intensity of autofluorescence of a substrate of the sensor chip were theoretically and experimentally evaluated to maximize the signal-to-noise ratio. As the result, an excitation wavelength of 390 nm was selected to excite SPR on an Al film of the sensor chip. The sandwich assay of norovirus VLPs was performed using the designed sensor. Minimum detectable concentration of 0.01 ng/mL, which corresponds to 100 virus-like particles included in the detection region of the V-trench, was demonstrated.
UR - http://www.scopus.com/inward/record.url?scp=84994496282&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84994496282&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2016.08.099
DO - 10.1016/j.bios.2016.08.099
M3 - Article
C2 - 27597126
AN - SCOPUS:84994496282
SN - 0956-5663
VL - 93
SP - 260
EP - 266
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -