Detection of pre-mRNA splicing in vitro by an RNA-templated fluorogenic reaction

Yasutsugu Tamura, Kazuhiro Furukawa, Rei Yoshimoto, Yuto Kawai, Minoru Yoshida, Satoshi Tsuneda, Yoshihiro Ito, Hiroshi Abe

研究成果: Article

8 引用 (Scopus)

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RNA splicing is an important target for basic research of disease mechanisms and for drug discovery. Here, we report a new method for analysis of the in vitro RNA splicing process that produces fluorescence using a reduction-triggered fluorescence (RETF) probe. The fluorescence signal is produced only when the two probes bind side-by-side with a specific RNA target. Precursor messenger RNA and mature messenger RNA originating from the chicken δ-crystallin (CDC) gene were successfully discriminated in solution using an RETF probe with the assistance of helper oligonucleotide strands. Also, we successfully applied RETF probes to the detection of emerging mature mRNA in an in vitro splicing process.

元の言語English
ページ(範囲)7248-7251
ページ数4
ジャーナルBioorganic and Medicinal Chemistry Letters
22
発行部数23
DOI
出版物ステータスPublished - 01-12-2012

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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