Determination of 3-hydroxyisovalerylcarnitine and other acylcarnitine levels using liquid chromatography-tandem mass spectrometry in serum and urine of a patient with multiple carboxylase deficiency

Yasuhiro Maeda; Tetsuya Ito; Hironori Ohmi; Kyoko Yokoi; Yoko Nakajima; Akihito Ueta; Yukihisa Kurono; Hajime Togari; Naruji Sugiyama

doi:10.1016/j.jchromb.2007.11.037

Determination of 3-hydroxyisovalerylcarnitine and other acylcarnitine levels using liquid chromatography-tandem mass spectrometry in serum and urine of a patient with multiple carboxylase deficiency

Yasuhiro Maeda
, Tetsuya Ito
, Hironori Ohmi
, Kyoko Yokoi
, Yoko Nakajima
, Akihito Ueta
, Yukihisa Kurono
, Hajime Togari
, Naruji Sugiyama

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

35 被引用数 (Scopus)

抄録

Due to its increased concentration in blood, 3-hydroxyisovalerylcarnitine (C5OH-I) is an important indicator for the diagnosis of organic acidemias in newborns. However, C5OH-I has not been used as a standard in tandem mass spectrometric (MS/MS) assays because its isolation is difficult. We developed a new synthesis of C5OH-I and investigated its behavior by MS/MS. A method using the multiple reaction monitoring (MRM) mode of MS/MS with HPLC was developed which provides high accuracy, precision and reproducibility. Acylcarnitine profiles in the serum and urine of a patient with multiple carboxylase deficiency (MCD) showed increased levels compared to a healthy patient.

本文言語	英語
ページ（範囲）	154-159
ページ数	6
ジャーナル	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
巻	870
号	2
DOI	https://doi.org/10.1016/j.jchromb.2007.11.037
出版ステータス	出版済み - 15-07-2008
外部発表	はい

All Science Journal Classification (ASJC) codes

分析化学
生化学
臨床生化学
細胞生物学

文献へのアクセス

10.1016/j.jchromb.2007.11.037

他のファイルとリンク

フィンガープリント

「Determination of 3-hydroxyisovalerylcarnitine and other acylcarnitine levels using liquid chromatography-tandem mass spectrometry in serum and urine of a patient with multiple carboxylase deficiency」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル

Maeda, Y., Ito, T., Ohmi, H., Yokoi, K., Nakajima, Y., Ueta, A., Kurono, Y., Togari, H., & Sugiyama, N. (2008). Determination of 3-hydroxyisovalerylcarnitine and other acylcarnitine levels using liquid chromatography-tandem mass spectrometry in serum and urine of a patient with multiple carboxylase deficiency. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 870(2), 154-159. https://doi.org/10.1016/j.jchromb.2007.11.037

Maeda, Yasuhiro ; Ito, Tetsuya ; Ohmi, Hironori その他. / Determination of 3-hydroxyisovalerylcarnitine and other acylcarnitine levels using liquid chromatography-tandem mass spectrometry in serum and urine of a patient with multiple carboxylase deficiency. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2008 ; Vol. 870, No. 2. pp. 154-159.

@article{e68b7a18017849028d6a9586500b65a5,

title = "Determination of 3-hydroxyisovalerylcarnitine and other acylcarnitine levels using liquid chromatography-tandem mass spectrometry in serum and urine of a patient with multiple carboxylase deficiency",

abstract = "Due to its increased concentration in blood, 3-hydroxyisovalerylcarnitine (C5OH-I) is an important indicator for the diagnosis of organic acidemias in newborns. However, C5OH-I has not been used as a standard in tandem mass spectrometric (MS/MS) assays because its isolation is difficult. We developed a new synthesis of C5OH-I and investigated its behavior by MS/MS. A method using the multiple reaction monitoring (MRM) mode of MS/MS with HPLC was developed which provides high accuracy, precision and reproducibility. Acylcarnitine profiles in the serum and urine of a patient with multiple carboxylase deficiency (MCD) showed increased levels compared to a healthy patient.",

author = "Yasuhiro Maeda and Tetsuya Ito and Hironori Ohmi and Kyoko Yokoi and Yoko Nakajima and Akihito Ueta and Yukihisa Kurono and Hajime Togari and Naruji Sugiyama",

note = "Funding Information: This research was supported by Grant-in-Aid for Research in Nagoya City University.",

year = "2008",

month = jul,

day = "15",

doi = "10.1016/j.jchromb.2007.11.037",

language = "English",

volume = "870",

pages = "154--159",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

issn = "1570-0232",

publisher = "Elsevier BV",

number = "2",

}

Maeda, Y , Ito, T, Ohmi, H, Yokoi, K, Nakajima, Y, Ueta, A, Kurono, Y, Togari, H & Sugiyama, N 2008, 'Determination of 3-hydroxyisovalerylcarnitine and other acylcarnitine levels using liquid chromatography-tandem mass spectrometry in serum and urine of a patient with multiple carboxylase deficiency', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 870, no. 2, pp. 154-159. https://doi.org/10.1016/j.jchromb.2007.11.037

Determination of 3-hydroxyisovalerylcarnitine and other acylcarnitine levels using liquid chromatography-tandem mass spectrometry in serum and urine of a patient with multiple carboxylase deficiency. / Maeda, Yasuhiro ; Ito, Tetsuya; Ohmi, Hironori その他.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 870, No. 2, 15.07.2008, p. 154-159.

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

TY - JOUR

T1 - Determination of 3-hydroxyisovalerylcarnitine and other acylcarnitine levels using liquid chromatography-tandem mass spectrometry in serum and urine of a patient with multiple carboxylase deficiency

AU - Maeda, Yasuhiro

AU - Ito, Tetsuya

AU - Ohmi, Hironori

AU - Yokoi, Kyoko

AU - Nakajima, Yoko

AU - Ueta, Akihito

AU - Kurono, Yukihisa

AU - Togari, Hajime

AU - Sugiyama, Naruji

N1 - Funding Information: This research was supported by Grant-in-Aid for Research in Nagoya City University.

PY - 2008/7/15

Y1 - 2008/7/15

N2 - Due to its increased concentration in blood, 3-hydroxyisovalerylcarnitine (C5OH-I) is an important indicator for the diagnosis of organic acidemias in newborns. However, C5OH-I has not been used as a standard in tandem mass spectrometric (MS/MS) assays because its isolation is difficult. We developed a new synthesis of C5OH-I and investigated its behavior by MS/MS. A method using the multiple reaction monitoring (MRM) mode of MS/MS with HPLC was developed which provides high accuracy, precision and reproducibility. Acylcarnitine profiles in the serum and urine of a patient with multiple carboxylase deficiency (MCD) showed increased levels compared to a healthy patient.

AB - Due to its increased concentration in blood, 3-hydroxyisovalerylcarnitine (C5OH-I) is an important indicator for the diagnosis of organic acidemias in newborns. However, C5OH-I has not been used as a standard in tandem mass spectrometric (MS/MS) assays because its isolation is difficult. We developed a new synthesis of C5OH-I and investigated its behavior by MS/MS. A method using the multiple reaction monitoring (MRM) mode of MS/MS with HPLC was developed which provides high accuracy, precision and reproducibility. Acylcarnitine profiles in the serum and urine of a patient with multiple carboxylase deficiency (MCD) showed increased levels compared to a healthy patient.

UR - https://www.scopus.com/pages/publications/47249124537

UR - https://www.scopus.com/pages/publications/47249124537#tab=citedBy

U2 - 10.1016/j.jchromb.2007.11.037

DO - 10.1016/j.jchromb.2007.11.037

M3 - Article

C2 - 18088573

AN - SCOPUS:47249124537

SN - 1570-0232

VL - 870

SP - 154

EP - 159

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 2

ER -

Maeda Y , Ito T, Ohmi H, Yokoi K, Nakajima Y, Ueta A その他. Determination of 3-hydroxyisovalerylcarnitine and other acylcarnitine levels using liquid chromatography-tandem mass spectrometry in serum and urine of a patient with multiple carboxylase deficiency. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2008 7月 15;870(2):154-159. doi: 10.1016/j.jchromb.2007.11.037