Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA

Nobuya Kitaguchi, Yasuo Tokushima, Kiyomi Oishi, Yasuyuki Takahashi, Satoshi Shiojiri, Shigenobu Nakamura, Seigo Tanaka, Ryoji Kodaira, Hirataka Ito

研究成果: Article査読

39 被引用数 (Scopus)

抄録

β-Amyloid protein precursors (APP) having proteinase inhibitor domains (APPI) were quantified by a new sandwich enzyme linked immunosorbent assay for detection of active (free) form of proteinase inhibitors by using trypsin in place of the first antibody and by denaturation of APPI-trypsin complex in the microtiterplate. The concentration of APPs having APPI in cerebrospinal fluid of Alzheimer's disease patients was found, by this method, to be significantly elevated compared with those of multi-infarct dementia.

本文言語English
ページ(範囲)1453-1459
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
166
3
DOI
出版ステータスPublished - 14-02-1990

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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