Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection

Ziyu Song, Dan Ge, Kana Ishii, Hiroshi Yamada, Kazuya Toriumi, Hiroyuki Watanabe, Toshitaka Nabeshima, Takeshi Fukushima

研究成果: Article

8 引用 (Scopus)

抜粋

The concentration of brain N-acetylaspartic acid (NAA) in mice was determined by high-performance liquid chromatography (HPLC) using fluorescence detection after pre-column derivatization with 4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benzoxadiazole (DBD-ED). Six different brain parts, namely, the prefrontal cortex, olfactory bulb, nucleus accumbens, striatum, cerebellum and hippocampus, of male C57BL6/J mice, were investigated. The NAA concentration (nmol/mg protein) was highest in the olfactory bulb (58.2±4.0, n=8) and lowest in the hippocampus (42.8±1.6, n=8). The proposed HPLC method with fluorescence detection was successfully used to determine the NAA concentration in each investigated brain area.

元の言語English
ページ(範囲)147-151
ページ数5
ジャーナルBiomedical Chromatography
26
発行部数2
DOI
出版物ステータスPublished - 01-02-2012
外部発表Yes

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

これを引用

Song, Z., Ge, D., Ishii, K., Yamada, H., Toriumi, K., Watanabe, H., Nabeshima, T., & Fukushima, T. (2012). Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection. Biomedical Chromatography, 26(2), 147-151. https://doi.org/10.1002/bmc.1639