Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection

Ziyu Song; Dan Ge; Kana Ishii; Hiroshi Yamada; Kazuya Toriumi; Hiroyuki Watanabe; Toshitaka Nabeshima; Takeshi Fukushima

doi:10.1002/bmc.1639

Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection

Ziyu Song
, Dan Ge
, Kana Ishii
, Hiroshi Yamada
, Kazuya Toriumi
, Hiroyuki Watanabe
, Toshitaka Nabeshima
, Takeshi Fukushima

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

10 被引用数 (Scopus)

抄録

The concentration of brain N-acetylaspartic acid (NAA) in mice was determined by high-performance liquid chromatography (HPLC) using fluorescence detection after pre-column derivatization with 4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benzoxadiazole (DBD-ED). Six different brain parts, namely, the prefrontal cortex, olfactory bulb, nucleus accumbens, striatum, cerebellum and hippocampus, of male C57BL6/J mice, were investigated. The NAA concentration (nmol/mg protein) was highest in the olfactory bulb (58.2±4.0, n=8) and lowest in the hippocampus (42.8±1.6, n=8). The proposed HPLC method with fluorescence detection was successfully used to determine the NAA concentration in each investigated brain area.

本文言語	英語
ページ（範囲）	147-151
ページ数	5
ジャーナル	Biomedical Chromatography
巻	26
号	2
DOI	https://doi.org/10.1002/bmc.1639
出版ステータス	出版済み - 02-2012
外部発表	はい

All Science Journal Classification (ASJC) codes

分析化学
生化学
分子生物学
薬理学
創薬
臨床生化学

文献へのアクセス

10.1002/bmc.1639

他のファイルとリンク

引用スタイル

@article{681a5bd39f0742ef882346263ec8a458,

title = "Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection",

abstract = "The concentration of brain N-acetylaspartic acid (NAA) in mice was determined by high-performance liquid chromatography (HPLC) using fluorescence detection after pre-column derivatization with 4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benzoxadiazole (DBD-ED). Six different brain parts, namely, the prefrontal cortex, olfactory bulb, nucleus accumbens, striatum, cerebellum and hippocampus, of male C57BL6/J mice, were investigated. The NAA concentration (nmol/mg protein) was highest in the olfactory bulb (58.2±4.0, n=8) and lowest in the hippocampus (42.8±1.6, n=8). The proposed HPLC method with fluorescence detection was successfully used to determine the NAA concentration in each investigated brain area.",

author = "Ziyu Song and Dan Ge and Kana Ishii and Hiroshi Yamada and Kazuya Toriumi and Hiroyuki Watanabe and Toshitaka Nabeshima and Takeshi Fukushima",

year = "2012",

month = feb,

doi = "10.1002/bmc.1639",

language = "English",

volume = "26",

pages = "147--151",

journal = "Biomedical Chromatography",

issn = "0269-3879",

publisher = "John Wiley and Sons Ltd",

number = "2",

}

TY - JOUR

T1 - Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection

AU - Song, Ziyu

AU - Ge, Dan

AU - Ishii, Kana

AU - Yamada, Hiroshi

AU - Toriumi, Kazuya

AU - Watanabe, Hiroyuki

AU - Nabeshima, Toshitaka

AU - Fukushima, Takeshi

PY - 2012/2

Y1 - 2012/2

N2 - The concentration of brain N-acetylaspartic acid (NAA) in mice was determined by high-performance liquid chromatography (HPLC) using fluorescence detection after pre-column derivatization with 4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benzoxadiazole (DBD-ED). Six different brain parts, namely, the prefrontal cortex, olfactory bulb, nucleus accumbens, striatum, cerebellum and hippocampus, of male C57BL6/J mice, were investigated. The NAA concentration (nmol/mg protein) was highest in the olfactory bulb (58.2±4.0, n=8) and lowest in the hippocampus (42.8±1.6, n=8). The proposed HPLC method with fluorescence detection was successfully used to determine the NAA concentration in each investigated brain area.

AB - The concentration of brain N-acetylaspartic acid (NAA) in mice was determined by high-performance liquid chromatography (HPLC) using fluorescence detection after pre-column derivatization with 4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benzoxadiazole (DBD-ED). Six different brain parts, namely, the prefrontal cortex, olfactory bulb, nucleus accumbens, striatum, cerebellum and hippocampus, of male C57BL6/J mice, were investigated. The NAA concentration (nmol/mg protein) was highest in the olfactory bulb (58.2±4.0, n=8) and lowest in the hippocampus (42.8±1.6, n=8). The proposed HPLC method with fluorescence detection was successfully used to determine the NAA concentration in each investigated brain area.

UR - https://www.scopus.com/pages/publications/84855428466

UR - https://www.scopus.com/pages/publications/84855428466#tab=citedBy

U2 - 10.1002/bmc.1639

DO - 10.1002/bmc.1639

M3 - Article

C2 - 21547934

AN - SCOPUS:84855428466

SN - 0269-3879

VL - 26

SP - 147

EP - 151

JO - Biomedical Chromatography

JF - Biomedical Chromatography

IS - 2

ER -

Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection

抄録

All Science Journal Classification (ASJC) codes

文献へのアクセス

他のファイルとリンク

フィンガープリント

引用スタイル