TY - JOUR
T1 - Determination of tetrahydrobiopterin in murine locus coeruleus by HPLC with fluorescence detection
AU - Kaneko, Yoko S.
AU - Mori, Keiji
AU - Nakashima, Akira
AU - Nagatsu, Ikuko
AU - Nagatsu, Toshiharu
AU - Ota, Akira
N1 - Funding Information:
This work was supported by grants-in-aid from Fujita Health University, Japan, to AO and YSK. We thank Ms. Hiromi Hasegawa, Chisako Nakagawa, Mayumi Hirashita, and Naomi Yazawa for their technical help.
PY - 2001
Y1 - 2001
N2 - Tetrahydrobiopterin in the murine locus coeruleus was measured as its fully oxidized form, biopterin, using a HPLC coupled to a fluorescence detector, because tetrahydrobiopterin itself cannot be detected by such means. The differential oxidization method distinguished tetrahydrobiopterin-derived biopterin and dihydrobiopterin-derived biopterin. The protocol reported here is a rapid and sensitive method that facilitates the measurement of tissue and/or cellular tetrahydrobiopterin. Using this assay protocol, we were able to detect and quantify variations in the tetrahydrobiopterin content in the murine locus coeruleus.
AB - Tetrahydrobiopterin in the murine locus coeruleus was measured as its fully oxidized form, biopterin, using a HPLC coupled to a fluorescence detector, because tetrahydrobiopterin itself cannot be detected by such means. The differential oxidization method distinguished tetrahydrobiopterin-derived biopterin and dihydrobiopterin-derived biopterin. The protocol reported here is a rapid and sensitive method that facilitates the measurement of tissue and/or cellular tetrahydrobiopterin. Using this assay protocol, we were able to detect and quantify variations in the tetrahydrobiopterin content in the murine locus coeruleus.
UR - https://www.scopus.com/pages/publications/0034887920
UR - https://www.scopus.com/pages/publications/0034887920#tab=citedBy
U2 - 10.1016/S1385-299X(01)00081-2
DO - 10.1016/S1385-299X(01)00081-2
M3 - Article
C2 - 11522525
AN - SCOPUS:0034887920
SN - 1385-299X
VL - 8
SP - 25
EP - 31
JO - Brain Research Protocols
JF - Brain Research Protocols
IS - 1
ER -