Developing novel methods to search for substrates of protein kinases such as Rho-kinase

Tomoki Nishioka, Md Hasanuzzaman Shohag, Mutsuki Amano, Kozo Kaibuchi

研究成果: Review article査読

12 被引用数 (Scopus)

抄録

Protein phosphorylation is a major and essential post-translational modification in eukaryotic cells that plays a critical role in various cellular processes. Recent progresses in mass spectrometry techniques have enabled the effective identification and analysis of protein phosphorylation. Mass spectrometry-based approaches in investigating protein phosphorylation are very powerful and informative and can further improve our understanding of protein phosphorylation as a whole, but they cannot determine the upstream kinases involved. We introduce several studies that attempted to uncover the relationships between various kinases of interest and substrates, including two methods we developed: an in vitro approach termed the kinase-interacting substrate screening (KISS) method and an in vivo approach termed the phosphatase inhibitor and kinase inhibitor substrate screening (PIKISS) method. This article is part of a Special Issue entitled: Inhibitors of Protein Kinases.

本文言語English
ページ(範囲)1663-1666
ページ数4
ジャーナルBiochimica et Biophysica Acta - Proteins and Proteomics
1854
10
DOI
出版ステータスPublished - 01-10-2015
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分析化学
  • 生物理学
  • 生化学
  • 分子生物学

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