Development and evaluation of novel loop-mediated isothermal amplification for rapid detection of bla(IMP-1) and bla(VIM-2) genes

Tadashi Kojima, Naohiro Shibata, Masanari Ikedo, Yoshichika Arakawa

研究成果: ジャーナルへの寄稿学術論文査読

2 被引用数 (Scopus)

抄録

Loop-mediated isothermal amplification (LAMP) amplifies a target gene with high specificity and rapidity under isothermal conditions. LAMP assays were developed for the rapid detection of metallo-beta-lactamase (MBL) genes such as bla(IMP-1)) and bla(VIM-2). We initially designed specific primers to detect MBL genes for LAMP assays and evaluated the specificity and sensitivity of these assays. LAMP assays amplified MBL genes under a constant temperature of 63 degrees C within 1 hour, and were compared to PCR in MBL-producing strains. The results of MBL genes typing by LAMP assays agree completely with PCR results. The lower detection limits of bla(IMP-1)- and bla(VIM-2)-LAMP assays using real-time turbidimeters were 30cfu/test and 3cfu/test. After amplification, products were directly observed by the naked eye with a fluorescent detection reagent. In conclusion, LAMP assays are convenient, rapid, and fully feasible for detecting MBL genes in ordinary clinical microbiology laboratories without special apparatus.

本文言語英語
ページ(範囲)405-412
ページ数8
ジャーナルKansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases
80
4
DOI
出版ステータス出版済み - 07-2006
外部発表はい

All Science Journal Classification (ASJC) codes

  • 医学一般

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