Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase

Keichiro Mihara, Chihaya Imai, Elaine Coustan-Smith, Jeffrey S. Dome, Massimo Dominici, Elio Vanin, Dario Campana

研究成果: Article査読

108 被引用数 (Scopus)

抄録

To create immortal mesenchymal cell lines, we transduced primary human bone marrow mesenchymal cells with telomerase reverse transcriptase (TERT). TERT+ mesenchymal cells continued to grow for > 2 years: parallel TERT- cultures underwent senescence after 15 weeks. TERT+ mesenchymal cells did not form foci in soft agar. had a normal karyotype and could differentiate into osteoblasts and chondrocytes. Their capacity to support leukaemic lymphoblasts and normal CD34+ haematopoietic cells was equal to or greater than that of primary cells; 42 TERT+ mesenchymal cell clones varied in their supporting capacity. Immortalized mesenchymal cells offer a promising tool for identifying molecules that regulate human haematopoiesis.

本文言語English
ページ(範囲)846-849
ページ数4
ジャーナルBritish Journal of Haematology
120
5
DOI
出版ステータスPublished - 01-03-2003
外部発表はい

All Science Journal Classification (ASJC) codes

  • 血液学

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