Development of 3RD generation immuno-pillar device for high sensitive detection of disease markers

We previously developed immuno-pillar devices for rapid and easy-to-use immunoassay [1-3]. However, improvement in the detection sensitivity (nM-pM) has still remained as a problem toward the screening test for disease markers with low cut-off values. We report here a third-generation immuno-pillar device in which the primary antibodies are covalently bounded to polymers on microbeads. By using this device we achieved pM-fM detection sensitivity of C-reactive protein (CRP) spiked in human serum. Moreover, we demonstrated that the device has long-term stability. From these results, it was proved that the third-generation device has capability required for practical use.