Technologies to develop scaffolds with controlled diameter and high porosity have great significance in tissue engineer ing. We have fabricated biodegradable 2D and 3D scaffol ds with ordered array of pores by casting polymer on self -assembled d-fructose (sugar) microspheres. First, ferrite mi croparticles were encapsulated in sugar spheres to make th em become magnetized. After sieving magnetic sugar partic les, those diameter-controlled particles were attracted by a magnet to form a self-assembled template for polymer cas ting. After removal of sugar particles, ordered array of po res were generated on the surface of sheet-like scaffolds. O rdered pores are also generated in 3D tubular scaffolds by using magnetic force and viscoelasticity of PVA solution. It was confirmed that MSPs leaching method makes it pos sible to make high porous scaffolds in spite of the thicknes s of scaffold and the porosity is homogeneous. Finally, the biocompatibility of the developed scaffold was confirmed by the viability of human umbilical vein endothelial cells.