Development of color micro optical-CT: Evaluation using phantom and biological samples

C. Murata, A. Teramoto, C. Kaneko, H. Fujita

研究成果: Conference contribution

抄録

Micro-optical computed tomography (MOCT) is a method for performing image reconstruction using microscopic images to obtain tomographic images of small samples. Compared with conventional observation methods, it offers the possibility to obtain tomograpic images without distortion, and create three-dimensional images. However, MOCT system which developed previously outputs monochrome images, while useful color information could not be obtained from the analysis of the sample. Therefore, we focused on the features that simplify the wavelength measurement of visible light, and developed a color MOCT system that can obtain color tomographic images. In this study, we acquired tomographic images of phantom and biological samples, and evaluated its usefulness. In this system, a digital single-lens reflex camera was used as a detector that was connected to a stereoscopic microscope, and projection images were obtained by rotating the sample. The sample was fixed in the test tube by carrageenan. The projection images were obtained from various projection angles followed by decomposing the R, G and B components. Subsequently, we performed image reconstruction for each component using filtered back projection. Finally, color tomographic image was obtained by combining the three-color component images. In the experiments, we scanned a color phantom and biological samples and evaluated the color and shape reproducibility. As a result, it was found that the color and shape of the tomographic images were similar to those of the samples. These results indicate that the proposed system may be useful to obtain the three-dimensional color structure of biological samples.

本文言語English
ホスト出版物のタイトルMedical Imaging 2015
ホスト出版物のサブタイトルBiomedical Applications in Molecular, Structural, and Functional Imaging
編集者Barjor Gimi, Robert C. Molthen
出版社SPIE
ISBN(電子版)9781628415070
DOI
出版ステータスPublished - 01-01-2015
イベントMedical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging - Orlando, United States
継続期間: 24-02-201526-02-2015

出版物シリーズ

名前Progress in Biomedical Optics and Imaging - Proceedings of SPIE
9417
ISSN(印刷版)1605-7422

Other

OtherMedical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging
CountryUnited States
CityOrlando
Period24-02-1526-02-15

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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