抄録
Recent studies using stem cells or cancer stem cells have revealed the importance of detecting minor populations of cells in blood or tissue and analyzing their biological characteristics. These cells can be separated from other cells by fluorescence-activated cell sorting (FACS) using antibodies that bind to surface antigen; however, the majority of these cells do not have specific surface antigens. Furthermore, the cells must be kept alive throughout the procedure to analyze their biological characteristics. To improve these limitations, we have established a novel laboratory test named mRNA quantification after fluorescence-activated cell sorting(FACS-mQ). Using this method, cells are sorted by a specific gene expression pattern, and the gene expression profile in the sorted cells can be easily analyzed. In this feature, we will introduce the typical applications of FACS-mQ targeting intracellular mRNA and intracellular nuclear antigens. These methods will hopefully contribute to the accumulation of knowledge regarding human stem cells, cancer stem cells, and small populations of cells, the biological characteristics of which are mostly unknown.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 139-144 |
| ページ数 | 6 |
| ジャーナル | Rinsho byori. The Japanese journal of clinical pathology |
| 巻 | 60 |
| 発行部数 | 2 |
| 出版物ステータス | Published - 01-02-2012 |
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All Science Journal Classification (ASJC) codes
- Medicine(all)
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[Development of mRNA quantification after fluorescence activated cell sorting]. / Yamada, Hiroya.
：: Rinsho byori. The Japanese journal of clinical pathology, 巻 60, 番号 2, 01.02.2012, p. 139-144.研究成果: Article
TY - JOUR
T1 - [Development of mRNA quantification after fluorescence activated cell sorting].
AU - Yamada, Hiroya
PY - 2012/2/1
Y1 - 2012/2/1
N2 - Recent studies using stem cells or cancer stem cells have revealed the importance of detecting minor populations of cells in blood or tissue and analyzing their biological characteristics. These cells can be separated from other cells by fluorescence-activated cell sorting (FACS) using antibodies that bind to surface antigen; however, the majority of these cells do not have specific surface antigens. Furthermore, the cells must be kept alive throughout the procedure to analyze their biological characteristics. To improve these limitations, we have established a novel laboratory test named mRNA quantification after fluorescence-activated cell sorting(FACS-mQ). Using this method, cells are sorted by a specific gene expression pattern, and the gene expression profile in the sorted cells can be easily analyzed. In this feature, we will introduce the typical applications of FACS-mQ targeting intracellular mRNA and intracellular nuclear antigens. These methods will hopefully contribute to the accumulation of knowledge regarding human stem cells, cancer stem cells, and small populations of cells, the biological characteristics of which are mostly unknown.
AB - Recent studies using stem cells or cancer stem cells have revealed the importance of detecting minor populations of cells in blood or tissue and analyzing their biological characteristics. These cells can be separated from other cells by fluorescence-activated cell sorting (FACS) using antibodies that bind to surface antigen; however, the majority of these cells do not have specific surface antigens. Furthermore, the cells must be kept alive throughout the procedure to analyze their biological characteristics. To improve these limitations, we have established a novel laboratory test named mRNA quantification after fluorescence-activated cell sorting(FACS-mQ). Using this method, cells are sorted by a specific gene expression pattern, and the gene expression profile in the sorted cells can be easily analyzed. In this feature, we will introduce the typical applications of FACS-mQ targeting intracellular mRNA and intracellular nuclear antigens. These methods will hopefully contribute to the accumulation of knowledge regarding human stem cells, cancer stem cells, and small populations of cells, the biological characteristics of which are mostly unknown.
UR - http://www.scopus.com/inward/record.url?scp=84866459247&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84866459247&partnerID=8YFLogxK
M3 - Article
C2 - 22568095
AN - SCOPUS:84866459247
VL - 60
SP - 139
EP - 144
JO - Rinsho byori. The Japanese journal of clinical pathology
JF - Rinsho byori. The Japanese journal of clinical pathology
SN - 0047-1860
IS - 2
ER -