Development of thymic microenvironments in vitro is oxygen-dependent and requires permanent presence of T-cell progenitors

Wilfred T.V. Germeraad, Hiroshi Kawamoto, Manami Itoi, Yufei Jiang, Takashi Amagai, Yoshimoto Katsura, Willem Van Ewijk

研究成果: Article査読

22 被引用数 (Scopus)

抄録

Development of a mature T-cell repertoire in the thymus depends on lympho-stromal interaction between thymocytes and stromal cells. To facilitate intercellular contact, the epithelium in the thymus has differentiated into a unique three-dimensionally (3D)-oriented network. Here we analyze factors influencing induction and maintenance of the 3D configuration of the epithelial network in fetal thymic lobes in vitro. We show that the 3D configuration of the thymic stroma depends on (a) the oxygen pressure in vitro and (b) permanent physical contact between stromal cells and developing thymocytes. This latter feature is demonstrated by incubation of fetal thymic lobes with deoxyguanosine (d-Guo), inducing a 2D-organized thymic stroma, with thymic cysts appearing. Reconstitution of d-Guo-treated lobes with a limited number of flow-sorted T-cell progenitors restores the 3D configuration of the thymic epithelium, but only at high oxygen pressure. This study underlines the plasticity of thymic epithelium and shows that the unique organization of the thymic epithelium is dependent on both oxygen and crosstalk signals derived from developing thymocytes.

本文言語English
ページ(範囲)1225-1235
ページ数11
ジャーナルJournal of Histochemistry and Cytochemistry
51
9
DOI
出版ステータスPublished - 01-09-2003
外部発表はい

All Science Journal Classification (ASJC) codes

  • 解剖学
  • 組織学

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